Multiplexed paper analytical device for quantification of metals using distance-based detection.
Multiplexed paper analytical device for quantification of metals using distance-based detection.
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DOI：
10.1039/c5lc00364d
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发表时间：
2015-07-07
期刊：
影响因子：
6.1
通讯作者：
Henry CS
中科院分区：
文献类型：
--
作者：
Cate DM;Noblitt SD;Volckens J;Henry CS
接触含有金属的气雾剂与人体几乎每个器官的不良健康后果有关。商业上可用的颗粒金属定量技术既费时又费力，而且价格昂贵；样品分析通常超过100美元。我们报告了一种简单的技术，基于基于距离的检测主题，使用微流控纸基分析设备来量化空气颗粒物中金属镍、铜和铁的浓度。纸衬底用于制造独立的、自定时的传感器，并且只需要一滴样品就可以操作。与纸张微流体中依赖光学仪器进行分析的其他比色方法不同，使用基于距离的检测，分析物根据比色反应的距离进行视觉量化，类似于温度计上的读数温度。为了证明这种方法的有效性，在单通道设备中分别测量了镍、铜和铁；据报道，镍、铜和铁的检测下限分别为0.1、0.1和0.05微克。实现了对所有三种金属的多重分析，镍、铜和铁的检出限分别为1、5和1微克。我们还通过使用现成的喷墨打印机打印比色试剂的浓度梯度，扩展了多分析物检测的动态范围。对于常见的干扰，显示了分析物的选择性。为了证明该方法的实用性，从认证的焊接烟尘样品中测量了镍、铜和铁；纸质传感器测量的水平与重量法测定的已知数值相匹配。
Exposure to metal-containing aerosols has been linked with adverse health outcomes for almost every organ in the human body. Commercially available techniques for quantifying particulate metals are time-intensive, laborious, and expensive; often sample analysis exceeds $100. We report a simple technique, based upon a distance-based detection motif, for quantifying metal concentrations of Ni, Cu, and Fe in airborne particulate matter using microfluidic paper-based analytical devices. Paper substrates are used to create sensors that are self-contained, self-timing, and require only a drop of sample for operation. Unlike other colorimetric approaches in paper microfluidics that rely on optical instrumentation for analysis, with distance-based detection, analyte is quantified visually based on the distance of a colorimetric reaction, similar to reading temperature on a thermometer. To demonstrate the effectiveness of this approach, Ni, Cu, and Fe were measured individually in single-channel devices; detection limits as low as 0.1, 0.1, and 0.05 µg were reported for Ni, Cu, and Fe. Multiplexed analysis of all three metals was achieved with detection limits of 1, 5, and 1 µg for Ni, Cu, and Fe. We also extended the dynamic range for multi-analyte detection by printing concentration gradients of colorimetric reagents using an off the shelf inkjet printer. Analyte selectivity was demonstrated for common interferences. To demonstrate utility of the method, Ni, Cu, and Fe were measured from samples of certified welding fume; levels measured with paper sensors matched known values determined gravimetrically.
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影响因子：
6.2
作者：
JACOBS, WD;YOE, JH
通讯作者：
YOE, JH
影响因子：
7.4
作者：
Abe, Koji;Suzuki, Koji;Citterio, Daniel
通讯作者：
Citterio, Daniel
影响因子：
7.4
作者：
Hossain, S. M. Zakir;Brennan, John D.
通讯作者：
Brennan, John D.
影响因子：
7.4
作者：
Hossain, S. M. Zakir;Luckham, Roger E.;Brennan, John D.
通讯作者：
Brennan, John D.
影响因子：
7.4
作者：
Hossain, S. M. Zakir;Luckham, Roger E.;Brennan, John D.
通讯作者：
Brennan, John D.