Channel nuclear pore protein 54 directs sexual differentiation and neuronal wiring of female reproductive behaviors in Drosophila.
Channel nuclear pore protein 54 directs sexual differentiation and neuronal wiring of female reproductive behaviors in Drosophila.
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DOI：
10.1186/s12915-021-01154-6
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发表时间：
2021-10-20
期刊：
影响因子：
5.4
通讯作者：
Soller M
中科院分区：
文献类型：
--
作者：
Nallasivan MP;Haussmann IU;Civetta A;Soller M
交配后雌性生殖行为和生理发生深刻变化。妊娠相关的生理和行为变化的控制在很大程度上是硬连线到大脑中，以保证生殖成功，但基因表达程序，直接神经元分化和电路布线在性别决定途径的末端，以应对交配在很大程度上是未知的。在果蝇中，雄性衍生的性肽在雌性中诱导的交配后反应是一个很好的模型，可以阐明复杂的先天行为是如何硬连接到大脑中的。在这里，我们使用遗传学的方法来进一步表征果蝇雌性的性肽反应的分子和细胞结构。筛选影响性肽敏感性的突变，我们确定了通道核孔蛋白Nup 54基因作为介导性肽反应的重要组成部分，具有可行的突变等位基因，导致无法产卵和降低性肽暴露后的感受性。Nup 54指导八个表达扒手的成年大脑神经元的正确布线，这些神经元是产卵所必需的，而额外的通道Nups也介导性别分化。与Nup与物种形成的联系一致，Nup 54启动子是快速进化的热点，启动子变体改变核质穿梭。这些结果暗示核孔功能性神经元布线的性别肽的反应和性分化作为一个反应的性冲突所产生的男性衍生的性别肽，以指导女性交配后的反应。在线版本包含补充材料，可通过10.1186/s12915-021-01154-6获得。
Female reproductive behaviors and physiology change profoundly after mating. The control of pregnancy-associated changes in physiology and behaviors are largely hard-wired into the brain to guarantee reproductive success, yet the gene expression programs that direct neuronal differentiation and circuit wiring at the end of the sex determination pathway in response to mating are largely unknown. In Drosophila, the post-mating response induced by male-derived sex-peptide in females is a well-established model to elucidate how complex innate behaviors are hard-wired into the brain. Here, we use a genetic approach to further characterize the molecular and cellular architecture of the sex-peptide response in Drosophila females. Screening for mutations that affect the sensitivity to sex-peptide, we identified the channel nuclear pore protein Nup54 gene as an essential component for mediating the sex-peptide response, with viable mutant alleles leading to the inability of laying eggs and reducing receptivity upon sex-peptide exposure. Nup54 directs correct wiring of eight adult brain neurons that express pickpocket and are required for egg-laying, while additional channel Nups also mediate sexual differentiation. Consistent with links of Nups to speciation, the Nup54 promoter is a hot spot for rapid evolution and promoter variants alter nucleo-cytoplasmic shuttling. These results implicate nuclear pore functionality to neuronal wiring underlying the sex-peptide response and sexual differentiation as a response to sexual conflict arising from male-derived sex-peptide to direct the female post-mating response. The online version contains supplementary material available at 10.1186/s12915-021-01154-6.
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影响因子：
12.3
作者：
Haussmann IU;White K;Soller M
通讯作者：
Soller M
影响因子：
64.5
作者：
Asahina K;Watanabe K;Duistermars BJ;Hoopfer E;González CR;Eyjólfsdóttir EA;Perona P;Anderson DJ
通讯作者：
Anderson DJ
影响因子：
64.8
作者：
通讯作者：
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影响因子：
9.2
作者：
Carvalho, GB;Kapahi, P;Benzer, S
通讯作者：
Benzer, S
Piwi与核孔处的染色质相互作用，并混合结合果蝇卵巢体细胞中的核转录本。
影响因子：
14.9
作者：
Ilyin AA;Ryazansky SS;Doronin SA;Olenkina OM;Mikhaleva EA;Yakushev EY;Abramov YA;Belyakin SN;Ivankin AV;Pindyurin AV;Gvozdev VA;Klenov MS;Shevelyov YY
通讯作者：
Shevelyov YY