Dissecting the first transcriptional divergence during human embryonic development.
Dissecting the first transcriptional divergence during human embryonic development.
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DOI：
10.1007/s12015-011-9301-3
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发表时间：
2012-03
影响因子：
4.8
通讯作者：
De Vos, John
中科院分区：
文献类型：
--
作者：
Bai, Qiang;Assou, Said;Haouzi, Delphine;Ramirez, Jean-Marie;Monzo, Cecile;Becker, Fabienne;Gerbal-Chaloin, Sabine;Hamamah, Samir;De Vos, John
滋养层细胞谱系在胚泡阶段早期被指定，导致滋养外胚层和内细胞团的多能细胞的出现。使用双mRNA扩增技术和比较与转录组数据的多能干细胞，胎盘，生殖器和成人组织，我们在这里报告一些基本的分子特征的人壁滋养外胚层。除了已知在胎盘中起作用的基因（CGA、PGF、ALPPL 2和ABCG 2）外，人滋养外胚层还强烈表达层粘连蛋白，如LAMA 1和GAGE癌症/睾丸基因。ABCG 2在滋养外胚层中的表达水平非常高，是胎盘中的7.9倍，这表明该基因在保护极早期胚胎免受外源性物质的影响方面发挥了重要作用。包括CCKBR和DNMT 3L在内的几个基因仅在滋养外胚层中特异性上调，表明滋养层细胞谱系与生发层谱系共享DNMT 3L表达的瞬时爆发。滋养外胚层核心转录调控回路由13个紧密相连的转录因子（CEBPA、GATA 2、GATA 3、GCM 1、KLF 5、MAFK、MSX 2、MXD 1、PPARD、PPARG、PPP 1 R13 L、TFAP 2C和TP 63）形成，被发现在滋养外胚层中被诱导并在胎盘中维持。该网络的诱导可以在体外滋养层分化模型中重现。本文的在线版本（doi：10.1007/s12015-011-9301-3）包含补充材料，可供授权用户使用。
The trophoblast cell lineage is specified early at the blastocyst stage, leading to the emergence of the trophectoderm and the pluripotent cells of the inner cell mass. Using a double mRNA amplification technique and a comparison with transcriptome data on pluripotent stem cells, placenta, germinal and adult tissues, we report here some essential molecular features of the human mural trophectoderm. In addition to genes known for their role in placenta (CGA, PGF, ALPPL2 and ABCG2), human trophectoderm also strongly expressed Laminins, such as LAMA1, and the GAGE Cancer/Testis genes. The very high level of ABCG2 expression in trophectoderm, 7.9-fold higher than in placenta, suggests a major role of this gene in shielding the very early embryo from xenobiotics. Several genes, including CCKBR and DNMT3L, were specifically up-regulated only in trophectoderm, indicating that the trophoblast cell lineage shares with the germinal lineage a transient burst of DNMT3L expression. A trophectoderm core transcriptional regulatory circuitry formed by 13 tightly interconnected transcription factors (CEBPA, GATA2, GATA3, GCM1, KLF5, MAFK, MSX2, MXD1, PPARD, PPARG, PPP1R13L, TFAP2C and TP63), was found to be induced in trophectoderm and maintained in placenta. The induction of this network could be recapitulated in an in vitro trophoblast differentiation model. The online version of this article (doi:10.1007/s12015-011-9301-3) contains supplementary material, which is available to authorized users.
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DOI：
10.1111/j.1600-0897.2010.00860.x
发表时间：
2010-10
期刊：
American journal of reproductive immunology (New York, N.Y. : 1989)
影响因子：
--
作者：
Black SG;Arnaud F;Palmarini M;Spencer TE
通讯作者：
Spencer TE
影响因子：
5.8
作者：
Al-Shahrour, F;Díaz-Uriarte, R;Dopazo, J
通讯作者：
Dopazo, J
影响因子：
6.1
作者：
Assou, Said;Anahory, Tal;Hamamah, Samir
通讯作者：
Hamamah, Samir
影响因子：
16
作者：
Barak, Y;Nelson, MC;Evans, RM
通讯作者：
Evans, RM
影响因子：
56.9
作者：
Bourc'his, D;Xu, GL;Bestor, TH
通讯作者：
Bestor, TH