Topographical variations of the strain-dependent zonal properties of tibial articular cartilage by microscopic MRI.
Topographical variations of the strain-dependent zonal properties of tibial articular cartilage by microscopic MRI.
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DOI：
10.3109/03008207.2014.894997
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发表时间：
2014-06
影响因子：
2.9
通讯作者：
Xia Y
中科院分区：
文献类型：
--
作者：
Lee JH;Badar F;Kahn D;Matyas J;Qu X;Chen CT;Xia Y
应用显微磁共振成像（μMRI）技术，观察了犬胫骨内侧关节软骨在不同载荷作用下的区域性变化。在17.6 μm的深度分辨率下，获得了总共70个标本的T2和T1弛豫图和GAG（糖胺聚糖）图像。此外，从组织测量体积机械模量和水含量。对于无载荷的情况，（43.6 ± 8.1 ms），绝对厚度（907.6 ± 187.9 μm）和含水量（63.3 ± 9.3%）明显低于魔角T2平均值（51.1 ± 8.5）ms，绝对厚度（1251.6 ± 218.4）μm，含水量（73.2 ± 5.6%）。然而，覆盖区的GAG（86.0 ± 15.3 mg/ml）显著高于未覆盖区的GAG（70.0 ± 8.8 mg/ml）。复杂的关系被发现在作为外部负载的功能的组织特性。浅表区的组织参数比放射区的相同属性变化更深刻。当与未覆盖区域中的相同参数相比时，覆盖区域中的组织参数变化不同。该项目证实了载荷引起的软骨分子分布和结构的变化既具有深度依赖性又具有地形分布性。对胫骨层的这种详细了解可以改善对软骨的细微软化的早期检测，软骨的细微软化最终将导致诸如骨关节炎的临床疾病。
The topographical variations of the zonal properties of canine articular cartilage over the medial tibia were evaluated as the function of external loading by microscopic magnetic resonance imaging (μMRI). T2 and T1 relaxation maps and GAG (glycosaminoglycan) images from a total of 70 specimens were obtained with and without the mechanical loading at 17.6 μm depth resolution. In addition, bulk mechanical modulus and water content were measured from the tissue. For the bulk without loading, the means of T2 at magic angle (43.6 ± 8.1 ms), absolute thickness (907.6 ± 187.9 μm) and water content (63.3 ± 9.3%) on the meniscus-covered area were significantly lower than the means of T2 at magic angle (51.1 ± 8.5 ms), absolute thickness (1251.6 ± 218.4 μm) and water content (73.2 ± 5.6%) on the meniscus-uncovered area. However GAG (86.0 ± 15.3 mg/ml) on the covered area was significantly higher than GAG (70.0 ± 8.8 mg/ml) on the uncovered area. Complex relationships were found in the tissue properties as the function of external loading. The tissue parameters in the superficial zone changed more profoundly than the same properties in the radial zone. The tissue parameters in the meniscus-covered areas changed differently when comparing with the same parameters in the uncovered areas. This project confirms that the load-induced changes in the molecular distribution and structure of cartilage are both depth-dependent and topographically distributed. Such detailed knowledge of the tibial layer could improve the early detection of the subtle softening of the cartilage that will eventually lead to the clinical diseases such as osteoarthritis.
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影响因子：
7
作者：
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