Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi.
Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi.
复制标题
菌根真菌菌丝从土壤中吸收磷的多模态相关成像和建模。
DOI：
10.1111/nph.17980
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发表时间：
2022-04
期刊：
影响因子：
9.4
通讯作者：
Roose, Tiina
中科院分区：
文献类型：
--
作者：
Keyes, Sam;van Veelen, Arjen;Fletcher, Dan McKay;Scotson, Callum;Koebernick, Nico;Petroselli, Chiara;Williams, Katherine;Ruiz, Siul;Cooper, Laura;Mayon, Robbie;Duncan, Simon;Dumont, Marc;Jakobsen, Iver;Oldroyd, Giles;Tkacz, Andrzej;Poole, Philip;Mosselmans, Fred;Borca, Camelia;Huthwelker, Thomas;Jones, David L.;Roose, Tiina
关键词：
磷（P）是植物生长所必需的。丛枝菌根真菌（AMF）通过从远离根部的来源获得P以换取碳来帮助其吸收。关于AMF如何定殖土壤孔隙空间知之甚少，AMF增强P吸收的模型验证不佳。我们使用同步加速器X射线计算机断层扫描来可视化土壤中的菌根，并结合建模对P、硫（S）和铝（Al）进行同步加速器X射线荧光/X射线吸收近边结构（XRF/XANES）元素映射。我们发现，AMF接种对其他土壤真菌的定殖有抑制作用，并确定了AMF和非菌根真菌的菌丝之间的结构和生长速度的差异。我们的研究结果表明，AMF与高P和低Al的区域共存，并且优先与有机型P物种相关联，而不是富Al无机P。我们发现AMF避免富Al区域作为P的来源。富硫区域被发现与较高的菌丝密度和增加的有机相关P池相关，而氧化S物种被发现靠近AMF菌丝。接近AMF的S氧化增加表明观察到的变化与微生物组相关。我们的实验验证模型导致AMF菌丝对P-吸收的估计比之前估计的速率低一个数量级-这一结果对植物-土壤-AMF相互作用的建模具有重要意义。
Phosphorus (P) is essential for plant growth. Arbuscular mycorrhizal fungi (AMF) aid its uptake by acquiring P from sources distant from roots in return for carbon. Little is known about how AMF colonise soil pore‐space, and models of AMF‐enhanced P‐uptake are poorly validated. We used synchrotron X‐ray computed tomography to visualize mycorrhizas in soil and synchrotron X‐ray fluorescence/X‐ray absorption near edge structure (XRF/XANES) elemental mapping for P, sulphur (S) and aluminium (Al) in combination with modelling. We found that AMF inoculation had a suppressive effect on colonisation by other soil fungi and identified differences in structure and growth rate between hyphae of AMF and nonmycorrhizal fungi. Our results showed that AMF co‐locate with areas of high P and low Al, and preferentially associate with organic‐type P species over Al‐rich inorganic P. We discovered that AMF avoid Al‐rich areas as a source of P. Sulphur‐rich regions were found to be correlated with higher hyphal density and an increased organic‐associated P‐pool, whilst oxidized S‐species were found close to AMF hyphae. Increased S oxidation close to AMF suggested the observed changes were microbiome‐related. Our experimentally‐validated model led to an estimate of P‐uptake by AMF hyphae that is an order of magnitude lower than rates previously estimated – a result with significant implications for the modelling of plant–soil–AMF interactions.
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影响因子：
5.2
作者：
Berruti A;Lumini E;Balestrini R;Bianciotto V
通讯作者：
Bianciotto V
影响因子：
7.4
作者：
Allen, James W.;Shachar-Hill, Yair
通讯作者：
Shachar-Hill, Yair
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
6.9
作者：
Daly KR;Keyes SD;Masum S;Roose T
通讯作者：
Roose T
影响因子：
6
作者：
Boswell, Graeme P.;Davidson, Fordyce A.
通讯作者：
Davidson, Fordyce A.