Resolution of a structural competition involving dimeric G-quadruplex and its C-rich complementary strand.
Resolution of a structural competition involving dimeric G-quadruplex and its C-rich complementary strand.
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DOI：
10.1093/nar/gkj421
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发表时间：
2006
影响因子：
14.9
通讯作者：
Gargallo, R
中科院分区：
文献类型：
--
作者：
Jaumot, J;Eritja, R;Tauler, R;Gargallo, R
报道了端粒DNA序列5‘-Tag GGT Tag GGT-3’及其互补的5‘ACC CTA Acc-3’的二聚体G-四链/双链竞争的解析。为了实现这一目标，通过分子吸收、分子荧光和圆二色光谱对两个序列和这些序列混合物的熔融实验进行了监测。使用分子信标技术进行分子荧光测量，其中5‘标记GGT标记GGT-3’序列在链的末端用荧光团和猝灭剂标记。通过多元曲线分辨对实验光谱数据进行了数学分析，从而可以计算溶液中存在的所有已分解结构(二聚体、反平行和平行G-四链、Watson-Crick双链和单链)的浓度分布和纯光谱。我们的结果表明，平行G-四链比反平行G-四链更稳定。当存在互补的富C链时，观察到G-四链体结构和Watson-Crick双链的混合物，其中双链是主要物种。除了熔融温度外，还根据浓度分布确定了平行/反平行G-四链平衡和G-四链/双链平衡的平衡常数。
The resolution of the dimeric intermolecular G-quadruplex/duplex competition of the telomeric DNA sequence 5′-TAG GGT TAG GGT-3′ and of its complementary 5′ ACC CTA ACC CTA-3′ is reported. To achieve this goal, melting experiments of both sequences and of the mixtures of these sequences were monitored by molecular absorption, molecular fluorescence and circular dichroism spectroscopies. Molecular fluorescence measurements were carried out using molecular beacons technology, in which the 5′-TAG GGT TAG GGT-3′ sequence was labelled with a fluorophore and a quencher at the ends of the strand. Mathematical analysis of experimental spectroscopic data was performed by means of multivariate curve resolution, allowing the calculation of concentration profiles and pure spectra of all resolved structures (dimeric antiparallel and parallel G-quadruplexes, Watson–Crick duplex and single strands) present in solution. Our results show that parallel G-quadruplex is more stable than antiparallel G-quadruplex. When the complementary C-rich strand is present, a mixture of both G-quadruplex structures and Watson–Crick duplex is observed, the duplex being the major species. In addition to melting temperatures, equilibrium constants for the parallel/antiparallel G-quadruplex equilibrium and for the G-quadruplex/duplex equilibrium were determined from the concentration profiles.
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影响因子：
2.9
作者：
MARKY, LA;BRESLAUER, KJ
通讯作者：
BRESLAUER, KJ
影响因子：
1.3
作者：
Miyoshi, D;Matsumura, S;Sugimoto, N
通讯作者：
Sugimoto, N
影响因子：
3.5
作者：
Miyoshi, D;Nakao, A;Sugimoto, N
通讯作者：
Sugimoto, N
影响因子：
14.9
作者：
Darby, RAJ;Sollogoub, M;Fox, KR
通讯作者：
Fox, KR
DOI：
10.1080/07391102.2003.10506922
发表时间：
2003-10-01
影响因子：
4.4
作者：
Jaumot, J;Aviñó, A;Gargallo, R
通讯作者：
Gargallo, R