m6A and miRNA jointly regulate the development of breast muscles in duck embryonic stages.
m6A and miRNA jointly regulate the development of breast muscles in duck embryonic stages.
复制标题
DOI：
10.3389/fvets.2022.933850
复制
发表时间：
2022
影响因子：
3.2
通讯作者：
中科院分区：
文献类型：
--
作者：
N6-甲基腺苷(M6A)是一种丰富的内源基因修饰产物，在动物的生长发育过程中起着重要的调节作用。近年来，m6A修饰被发现在骨骼肌中起着关键作用。然而，m6A基因的修饰是否有助于北京鸭胚胎胸肌的发育还没有被探索。为了探讨m6A在鸭胚胎胸肌发育中的作用，本研究对19日龄(E19)和27日龄(E27)的鸭胚胎胸肌进行了m6A测序和miRNA测序。在E19共鉴定出12,717个m6A峰，代表总共7,438个基因转录本。共鉴定出14,703个m6A峰，与E27的7,753个基因的转录产物重叠。比较E19和E27，我们鉴定出2347个差异m6A峰，它们与1,605个m6A修饰基因(MMG)重叠。基因本体论(GO)和京都基因和基因组百科全书(KEGG)分析表明，MMG在多种肌肉或脂肪相关途径中富含，这也是我们对差异表达基因(DEG)的分析结果。联合分析m6A-seq和rna-seq数据显示，与脂肪酸β氧化和骨骼肌发育相关的通路显著丰富，表明m6A修饰参与了脂肪沉积和骨骼肌发育的调节。在E19和E27期之间发现了90个上调和102个下调的miRNAs。通过对MMG和DEGS共有的基因和差异表达的miRNAs(DEM)靶基因的重叠分析，我们鉴定了6个M6A-mRNA调控的miRNAs。最后，我们发现M6A修饰可以调节脂肪沉积和骨骼肌发育。综上所述，我们的结果表明，m6A修饰是通过影响mRNAs和miRNAs的表达而对鸭胚胎胸肌发育和脂肪沉积起关键调节作用。这是第一个全面描述鸭转录组中m6A模式的研究。这些数据为未来旨在确定m6A修饰在脂肪沉积和肌肉生长中的潜在功能作用的工作提供了坚实的基础。
N6-methyladenosine (m6A) is an abundant internal mRNA modification and plays a crucial regulatory role in animal growth and development. In recent years, m6A modification has been found to play a key role in skeletal muscles. However, whether m6A modification contributes to embryonic breast muscle development of Pekin ducks has not been explored. To explore the role of m6A in embryonic breast muscle development of ducks, we performed m6A sequencing and miRNA sequencing for the breast muscle of duck embryos on the 19th (E19) and 27th (E27) days. A total of 12,717 m6A peaks were identified at E19, representing a total of 7,438 gene transcripts. A total of 14,703 m6A peaks were identified, which overlapped with the transcripts of 7,753 genes at E27. Comparing E19 and E27, we identified 2,347 differential m6A peaks, which overlapped with 1,605 m6A-modified genes (MMGs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that MMGs were enriched in multiple muscle- or fat-related pathways, which was also revealed from our analysis of differentially expressed genes (DEGs). Conjoint analysis of m6A-seq and RNA-seq data showed that pathways related to β-oxidation of fatty acids and skeletal muscle development were significantly enriched, suggesting that m6A modification is involved in the regulation of fat deposition and skeletal muscle development. There were 90 upregulated and 102 downregulated miRNAs identified between the E19 and E27 stages. Through overlapping analysis of genes shared by MMGs and DEGs and the targets of differentially expressed miRNAs (DEMs), we identified six m6A-mRNA-regulated miRNAs. Finally, we found that m6A modification can regulate fat deposition and skeletal muscle development. In conclusion, our results suggest that m6A modification is a key regulator for embryonic breast muscle development and fat deposition of ducks by affecting expressions of mRNAs and miRNAs. This is the first study to comprehensively characterize the m6A patterns in the duck transcriptome. These data provide a solid basis for future work aimed at determining the potential functional roles of m6A modification in adipose deposition and muscle growth.
登录
查看更多内容
影响因子：
0.4
作者：
Gu, L. H.;Xu, T. S.;Hou, S. S.
通讯作者：
Hou, S. S.
影响因子：
--
作者：
Chang, Rui;Huang, Zeyi;Zhao, Size;Zou, Ju;Li, Yukun;Tan, Sijie
通讯作者：
Tan, Sijie
影响因子：
9
作者：
Baek JH;Kim DH;Lee J;Kim SJ;Chun KH
通讯作者：
Chun KH
影响因子：
14.9
作者：
Betel D;Wilson M;Gabow A;Marks DS;Sander C
通讯作者：
Sander C
影响因子：
3.7
作者：
Gu L;Xu T;Huang W;Xie M;Sun S;Hou S
通讯作者：
Hou S