The Cell Cycle Checkpoint Regulator ATR Is Required for Internal Aluminum Toxicity-Mediated Root Growth Inhibition in Arabidopsis.
The Cell Cycle Checkpoint Regulator ATR Is Required for Internal Aluminum Toxicity-Mediated Root Growth Inhibition in Arabidopsis.
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细胞周期检查点调节器 ATR 是拟南芥内部铝毒性介导的根生长抑制所必需的
DOI：
10.3389/fpls.2018.00118
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发表时间：
2018
影响因子：
5.6
通讯作者：
Huang CF
中科院分区：
文献类型：
--
作者：
Zhang Y;Guo J;Chen M;Li L;Wang L;Huang CF
铝（Al）可以靶向根细胞的多个部位，包括细胞壁、质膜和共塑成分。先前的研究表明，细胞周期检查点调节因子（ATR）共济失调毛细血管扩张突变和rad3相关是Al毒性诱导的als3根生长抑制所必需的，并且共plastic组分DNA是Al毒性的重要靶点。然而，在其他铝敏感突变体中，是否需要通过atr调控途径监测DNA完整性来抑制铝诱导的根生长仍然未知。在本研究中，我们证明atr突变也可以挽救Al超敏反应和Al诱导的star1细胞周期阻滞，这支持了ALS3和star1共同参与拟南芥Al解毒的假设。然而，ATR突变不能挽救almt1或stop1的Al敏感表型，这两种表型在Al的外解毒机制中都存在缺陷。我们进一步发现ATR突变也可以挽救als1的Al超敏和Al诱导的静止中心（QC）分化。因此，我们的研究结果表明，atr调控的途径参与了拟南芥内部铝毒性介导的根系生长抑制的调节。
Aluminum (Al) can target multiple sites of root cells for toxicity, including the cell wall, the plasma membrane and symplastic components. Previous work revealed that the cell cycle checkpoint regulator (ATR) Ataxia Telangiectasia-mutated and Rad3-related is required for Al toxicity-induced root growth inhibition in als3 and that the symplastic component DNA is an important target site of Al for the toxicity. However, whether monitoring DNA integrity through ATR-regulated pathway is required for Al-induced root growth inhibition in other Al-sensitive mutants remains unknown. In this study, we demonstrated that the atr mutation could also rescue the Al hypersensitivity and Al-induced cell cycle arrest in star1, which supports the hypothesis that ALS3 and STAR1 function together to be involved in the detoxification of Al in Arabidopsis. However, mutation of ATR could not rescue the Al-sensitive phenotype of almt1 or stop1, both of which are defective in external detoxification mechanisms of Al. We further showed that the Al hypersensitivity and Al-induced quiescent center (QC) differentiation in als1 could also be rescued by the atr mutation. Therefore, our results suggest that ATR-regulated pathway is involved in the modulation of internal Al toxicity-mediated root growth inhibition in Arabidopsis.
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影响因子：
4.3
作者：
Larsen, Paul B.;Cancel, Jesse;Ochoa, Vanessa
通讯作者：
Ochoa, Vanessa
影响因子：
7.4
作者：
Jones, DL;Kochian, LV;Gilroy, S
通讯作者：
Gilroy, S
影响因子：
3.9
作者：
KARLIK, SJ;EICHHORN, GL
通讯作者：
EICHHORN, GL
影响因子：
7.4
作者：
Lazof, DB;Goldsmith, JG;Linton, RW
通讯作者：
Linton, RW
DOI：
10.1073/pnas.0700117104
发表时间：
2007-06-05
影响因子：
11.1
作者：
Iuchi, Satoshi;Koyama, Hiroyuki;Kobayashi, Masatomo
通讯作者：
Kobayashi, Masatomo