Preferential electrical coupling regulates neocortical lineage-dependent microcircuit assembly.
Preferential electrical coupling regulates neocortical lineage-dependent microcircuit assembly.
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优先电耦合调节新皮质谱系依赖性微电路组装
DOI：
10.1038/nature10958
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发表时间：
2012-05-02
期刊：
影响因子：
64.8
通讯作者：
Shi, Song-Hai
中科院分区：
文献类型：
--
作者：
Yu, Yong-Chun;He, Shuijin;Chen, She;Fu, Yinghui;Brown, Keith N.;Yao, Xing-Hua;Ma, Jian;Gao, Kate P.;Sosinsky, Gina E.;Huang, Kun;Shi, Song-Hai
放射状胶质细胞是发育中的新皮层中的主要神经祖细胞。单个放射状胶质祖细胞的连续不对称分裂产生许多姐妹兴奋性神经元，其沿着伸长的放射状胶质纤维迁移，导致个体发育柱的形成。此外，个体发育柱中的姐妹兴奋性神经元优先与彼此形成特定的化学突触，而不是与附近的非兄弟姐妹形成特定的化学突触。虽然这些发现提供了重要的洞察功能列在新皮层的出现，很少有人知道的基础上，这种谱系依赖性组装的兴奋性神经元微电路在单细胞分辨率。在这里，我们表明，放射状排列的姐妹兴奋性神经元之间的瞬时电耦合调节随后在新皮层中形成特定的化学突触。多电极全细胞记录表明，姐妹兴奋性神经元优先形成强的电耦合，而不是与相邻的非姐妹兴奋性神经元在出生后的早期阶段。这种优先耦合允许姐妹兴奋性神经元之间的选择性电通信，促进它们的动作电位产生和同步放电。有趣的是，虽然这种电通信在化学突触出现之前基本消失，但电通信的阻断会损害个体发育柱中姐妹兴奋性神经元之间随后形成的特定化学突触。这些结果表明，依赖于谱系的瞬态电耦合和精确的兴奋性神经元微电路在新皮层的组装之间有很强的联系。
Radial glial cells are the primary neural progenitor cells in the developing neocortex. Consecutive asymmetric divisions of individual radial glial progenitor cells produce a number of sister excitatory neurons that migrate along the elongated radial glial fibre, resulting in the formation of ontogenetic columns,,. Moreover, sister excitatory neurons in ontogenetic columns preferentially develop specific chemical synapses with each other rather than with nearby non-siblings. Although these findings provide crucial insight into the emergence of functional columns in the neocortex, little is known about the basis of this lineage-dependent assembly of excitatory neuron microcircuits at single-cell resolution. Here we show that transient electrical coupling between radially aligned sister excitatory neurons regulates the subsequent formation of specific chemical synapses in the neocortex. Multiple-electrode whole-cell recordings showed that sister excitatory neurons preferentially form strong electrical coupling with each other rather than with adjacent non-sister excitatory neurons during early postnatal stages. This preferential coupling allows selective electrical communication between sister excitatory neurons, promoting their action potential generation and synchronous firing. Interestingly, although this electrical communication largely disappears before the appearance of chemical synapses, blockade of the electrical communication impairs the subsequent formation of specific chemical synapses between sister excitatory neurons in ontogenetic columns. These results suggest a strong link between lineage-dependent transient electrical coupling and the assembly of precise excitatory neuron microcircuits in the neocortex.
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DOI：
10.1073/pnas.0608607103
发表时间：
2006-11-14
影响因子：
11.1
作者：
Du, Zhijun;Podsypanina, Katrina;Li, Yi
通讯作者：
Li, Yi
影响因子：
13.9
作者：
Kriegstein A;Alvarez-Buylla A
通讯作者：
Alvarez-Buylla A
影响因子：
10.5
作者：
Holland, EC;Hively, WP;Varmus, HE
通讯作者：
Varmus, HE
影响因子：
64.8
作者：
Dupont, E;Hanganu, IL;Luhmann, HJ
通讯作者：
Luhmann, HJ
影响因子：
3.7
作者：
Wang Y;Barakat A;Zhou H
通讯作者：
Zhou H