Electrotonic coupling between pyramidal neurons in the neocortex.
Electrotonic coupling between pyramidal neurons in the neocortex.
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DOI：
10.1371/journal.pone.0010253
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发表时间：
2010-04-26
期刊：
影响因子：
3.7
通讯作者：
Zhou H
中科院分区：
文献类型：
--
作者：
Wang Y;Barakat A;Zhou H
电紧张耦合（即，电突触或间隙连接）是神经元同步的基础，因此对于许多生理功能和病理性疾病至关重要。神经元间的电突触已被广泛研究。虽然锥体细胞（PC）之间的电紧张耦合的研究正在出现，特别是在海马，证据仍然是罕见的新皮层。因此，在电生理学、解剖学和突触学特性方面，新皮层中PC的电紧张耦合在很大程度上是未知的。使用多个膜片钳记录差分干涉对比红外视频显微镜（IR-DIC）可视化，组织化学染色，和三维计算机重建，电紧张耦合记录密切的PC之间，主要是在内侧前额叶皮层以及在视觉皮层区域的雪貂和大鼠。与神经元间隙连接相比，这些电紧张性耦合具有一些特殊的特征。PC之间的电紧张性耦合的记录概率极低;但连接电导显著高，允许动作电位（AP）的直接传输，甚至耦合神经元之间的紧张性放电。AP发射，因此完全同步耦合PC之间; Postjunctional AP和小穗交替膜电位的轻微变化后，Postjunctional小穗，特别是在高频率，被求和，并最终达到AP阈值火。锥体电紧张耦合的这些特性在很大程度上满足了神经元组装同步化的需要，正如模拟研究所预测的那样。因此，这表明，PC的电紧张耦合在新皮层神经元同步化的产生中起着独特的作用。
Electrotonic couplings (i.e., electrical synapses or gap junctions) are fundamental to neuronal synchronization, and thus essential for many physiological functions and pathological disorders. Interneuron electrical synapses have been studied intensively. Although studies on electrotonic couplings between pyramidal cells (PCs) are emerging, particularly in the hippocampus, evidence is still rare in the neocortex. The electrotonic coupling of PCs in the neocortex is therefore largely unknown in terms of electrophysiological, anatomical and synaptological properties. Using multiple patch-clamp recording with differential interference contrast infrared videomicroscopy (IR-DIC) visualization, histochemical staining, and 3D-computer reconstruction, electrotonic coupling was recorded between close PCs, mainly in the medial prefrontal cortex as well as in the visual cortical regions of ferrets and rats. Compared with interneuron gap junctions, these electrotonic couplings were characterized by several special features. The recording probability of an electrotonic coupling between PCs is extremely low; but the junctional conductance is notably high, permitting the direct transmission of action potentials (APs) and even tonic firing between coupled neurons. AP firing is therefore perfectly synchronized between coupled PCs; Postjunctional APs and spikelets alternate following slight changes of membrane potentials; Postjunctional spikelets, especially at high frequencies, are summated and ultimately reach AP-threshold to fire. These properties of pyramidal electrotonic couplings largely fill the needs, as predicted by simulation studies, for the synchronization of a neuronal assembly. It is therefore suggested that the electrotonic coupling of PCs plays a unique role in the generation of neuronal synchronization in the neocortex.
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