Filifactor alocis modulates human neutrophil antimicrobial functional responses.
Filifactor alocis modulates human neutrophil antimicrobial functional responses.
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DOI：
10.1111/cmi.12829
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发表时间：
2018-06
影响因子：
3.4
通讯作者：
Uriarte SM
中科院分区：
文献类型：
--
作者：
Edmisson JS;Tian S;Armstrong CL;Vashishta A;Klaes CK;Miralda I;Jimenez-Flores E;Le J;Wang Q;Lamont RJ;Uriarte SM
纤裂因子是牙周病的新病原。中性粒细胞是龈缝中主要的先天免疫细胞。在这项研究中，我们研究了嗜中性粒细胞抗菌功能的调节。非调理化和血清调理化的金黄梭菌均被中性粒细胞吞噬，但不能被有效清除。与调理金黄色葡萄球菌和fMLF相比，非调理或血清调理的金黄色葡萄球菌对中性粒细胞的攻击分别诱导了最小的细胞内和细胞外呼吸爆发反应。然而，用嗜中性粒细胞预处理或同时用嗜中性粒细胞攻击不影响随后对颗粒刺激的氧化反应，这表明不能触发呼吸反应仅局限于嗜中性粒细胞吞噬体。此外，虽然中性粒细胞吞噬活的或热灭活的F. alocis的效率相同，但与活的生物体相比，热灭活的F. alocis引起了更高的细胞内呼吸爆发反应，同时CD35（分泌囊泡的标记物）的表面表达降低。F. alocis吞噬体保持不成熟的原因分别是特异性颗粒和azurophil颗粒的招募延迟和减少。这些结果表明，F. alocis通过阻止细胞内ROS的产生以及特异性和azurophil颗粒向细菌吞噬体的募集来抵抗中性粒细胞的抗菌反应。
Filifactor alocis is a newly appreciated pathogen in periodontal diseases. Neutrophils are the predominant innate immune cell in the gingival crevice. In this study we examined modulation of human neutrophil antimicrobial functions by F. alocis. Both non-opsonized and serum opsonized F. alocis were engulfed by neutrophils, but were not efficiently eliminated. Challenge of neutrophils with either non-opsonized or serum opsonized F. alocis induced a minimal intracellular as well as extracellular respiratory burst response compared to opsonized Staphylococcus aureus and fMLF, respectively. However, pre-treatment or simultaneous challenge of neutrophils with F. alocis did not affect the subsequent oxidative response to a particulate stimulus, suggesting that the inability to trigger the respiratory response was only localized to F. alocis phagosomes. In addition, while neutrophils engulfed live or heat-killed F. alocis with the same efficiency, heat-killed F. alocis elicited a higher intracellular respiratory burst response compared to viable organisms, along with decreased surface expression of CD35, a marker of secretory vesicles. F. alocis phagosomes remained immature by delayed and reduced recruitment of specific and azurophil granules, respectively. These results suggest that F. alocis withstands neutrophil antimicrobial responses by preventing intracellular ROS production, along with specific and azurophil granule recruitment to the bacterial phagosome.
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影响因子：
3.7
作者：
Hajishengallis G
通讯作者：
Hajishengallis G
Keystone-Pathogen假设。
DOI：
10.1038/nrmicro2873
发表时间：
2012-10
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
15.9
作者：
Hajishengallis G;Lamont RJ
通讯作者：
Lamont RJ
影响因子：
3.1
作者：
Darveau, Richard P.
通讯作者：
Darveau, Richard P.
影响因子：
--
作者：
Dahlén, G;Leonhardt, Å
通讯作者：
Leonhardt, Å