Allelic diversity in the transcriptomes of contrasting rust-infected genotypes of Lathyrus sativus, a lasting resource for smart breeding.
Allelic diversity in the transcriptomes of contrasting rust-infected genotypes of Lathyrus sativus, a lasting resource for smart breeding.
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DOI：
10.1186/s12870-014-0376-2
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发表时间：
2014-12-19
影响因子：
5.3
通讯作者：
Vaz Patto MC
中科院分区：
文献类型：
--
作者：
Almeida NF;Leitão ST;Krezdorn N;Rotter B;Winter P;Rubiales D;Vaz Patto MC
草豆（Lathyrus sativus L.）是一种具有潜在持久部分抗锈性的宝贵资源。为了深入了解抗性机制并鉴定潜在的抗性基因，我们通过RNA-seq技术首次从一种易感和部分抗锈病的草豌豆基因型的对照和接种了尿霉菌的草豌豆叶片中获得了全面的转录组组装。两个文库共有134,914个contigs，用于分析它们对锈病感染反应的差异表达。功能注释将植物数据库中60.4%的contigs（占总数的37.8%）归为33个主要功能类别，其中以“蛋白质”、“RNA”、“信号传导”、“运输”和“胁迫”最具代表性。转录谱显示，在主要植物激素信号通路的调控上存在相当大的差异：抗性基因型中水杨酸和脱落酸通路上调，而敏感基因型中茉莉酸和乙烯通路下调。作为潜在的抗性基因，我们鉴定了一个抗霉位点O （MLO）样基因和MLO相关转录物。在抗性基因型中，一些致病相关基因被上调，而在敏感基因型中，一些致病相关基因被下调。在两个接种文库中发现的病原体效应物，如锈病Rtp1转录物，可能负责防御相关转录物的下调。两个基因型共包含4892个多态性contigs， snp在不同功能类别间分布不均匀。蛋白质降解（29.7%）和信号受体激酶（8.2%）分化最大，说明了草豆对宿主/病原体军备竞赛的进化适应。我们在这里展示的大量新的、与抗性相关的基因组信息为这种迄今为止研究不足的、有价值的豆类作物的未来智能育种方法提供了非常宝贵的资源。本文的在线版本（doi:10.1186/s12870-014-0376-2）包含补充材料，可供授权用户使用。
Grass pea (Lathyrus sativus L.) is a valuable resource for potentially durable partial resistance to rust. To gain insight into the resistance mechanism and identify potential resistance genes, we generated the first comprehensive transcriptome assemblies from control and Uromyces pisi inoculated leafs of a susceptible and a partially rust-resistant grass pea genotype by RNA-seq. 134,914 contigs, shared by both libraries, were used to analyse their differential expression in response to rust infection. Functional annotation grouped 60.4% of the contigs present in plant databases (37.8% of total) to 33 main functional categories, being “protein”, “RNA”, “signalling”, “transport” and “stress” the most represented. Transcription profiles revealed considerable differences in regulation of major phytohormone signalling pathways: whereas Salicylic and Abscisic Acid pathways were up-regulated in the resistant genotype, Jasmonate and Ethylene pathways were down-regulated in the susceptible one. As potential Resistance-genes we identified a mildew resistance locus O (MLO)-like gene, and MLO-related transcripts. Also, several pathogenesis-related genes were up-regulated in the resistant and exclusively down regulated in the susceptible genotype. Pathogen effectors identified in both inoculated libraries, as e.g. the rust Rtp1 transcript, may be responsible for the down-regulation of defence-related transcripts. The two genotypes contained 4,892 polymorphic contigs with SNPs unevenly distributed between different functional categories. Protein degradation (29.7%) and signalling receptor kinases (8.2%) were the most diverged, illustrating evolutionary adaptation of grass pea to the host/pathogens arms race. The vast array of novel, resistance-related genomic information we present here provides a highly valuable resource for future smart breeding approaches in this hitherto under-researched, valuable legume crop. The online version of this article (doi:10.1186/s12870-014-0376-2) contains supplementary material, which is available to authorized users.
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影响因子：
3.2
作者：
Del Mar Rojas-Molina, Maria;Rubiales, Diego;Sillero, Josefina Carmen
通讯作者：
Sillero, Josefina Carmen
影响因子：
1.9
作者：
Barilli, E.;Satovic, Z.;Torres, A. M.
通讯作者：
Torres, A. M.
影响因子：
1.5
作者：
Barilli, Eleonora;Sillero, Josefina C.;Rubiales, Diego
通讯作者：
Rubiales, Diego
影响因子：
4.9
作者：
Fernandez, Diana;Tisserant, Emilie;Duplessis, Sebastien
通讯作者：
Duplessis, Sebastien
影响因子：
1.5
作者：
Barilli, Eleonora;Satovic, Zlatko;Torres, Ana M.
通讯作者：
Torres, Ana M.