Spacing requirements for Class I transcription activation in bacteria are set by promoter elements.
Spacing requirements for Class I transcription activation in bacteria are set by promoter elements.
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细菌中 I 类转录激活的间距要求由启动子元件设定。
DOI：
10.1093/nar/gku625
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发表时间：
2014-08
影响因子：
14.9
通讯作者：
Wang YP
中科院分区：
文献类型：
--
作者：
Zhou Y;Kolb A;Busby SJ;Wang YP
大肠杆菌cAMP受体蛋白（CRP）通过与上游核心启动子元件结合并与RNA聚合酶α亚基的c端结构域相互作用，激活许多启动子的转录起始。先前的研究表明，CRP的转录激活需要严格的间隔。在这里，我们报告，这种严格性可以改变的性质不同的启动子元件在目标启动子。将CRP转移到不同的上游位置，构建了几个系列的CRP依赖启动子，并测定了它们的活性。结果表明：(i)一个完整的UP元件，位于CRP DNA位点的下游，放松了激活的间距要求，增加了RNAP的招募和开放复合物的形成；（ii）远端UP亚位点在这种松弛中起关键作用；（iii）扩展- 10元件的修改也影响了依赖于crp的活化的间距要求。从这些结果中，我们得出结论，根据不同启动子元件的序列，依赖于CRP的转录激活的间距要求不同，我们的结果对于理解许多不同细菌中启动子的组织具有重要意义，这些启动子由使用类似CRP的激活机制的转录因子控制。
The Escherichia coli cAMP receptor protein (CRP) activates transcription initiation at many promoters by binding upstream of core promoter elements and interacting with the C-terminal domain of the RNA polymerase α subunit. Previous studies have shown stringent spacing is required for transcription activation by CRP. Here we report that this stringency can be altered by the nature of different promoter elements at target promoters. Several series of CRP-dependent promoters were constructed with CRP moved to different upstream locations, and their activities were measured. The results show that (i) a full UP element, located immediately downstream of the DNA site for CRP, relaxes the spacing requirements for activation and increases the recruitment of RNAP and open complex formation; (ii) the distal UP subsite plays the key role in this relaxation; (iii) modification of the extended −10 element also affects the spacing requirements for CRP-dependent activation. From these results, we conclude that the spacing requirements for CRP-dependent transcription activation vary according to the sequence of different promoter elements, and our results are important for understanding the organization of promoters in many different bacteria which are controlled by transcription factors that use activatory mechanisms similar to CRP.
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影响因子：
3.7
作者：
Shimada T;Fujita N;Yamamoto K;Ishihama A
通讯作者：
Ishihama A
影响因子：
64.5
作者：
GASTON, K;BELL, A;BUSBY, S
通讯作者：
BUSBY, S
DOI：
10.1126/science.1227786
发表时间：
2012-11-23
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Zhang Y;Feng Y;Chatterjee S;Tuske S;Ho MX;Arnold E;Ebright RH
通讯作者：
Ebright RH
DOI：
10.1073/pnas.0506687102
发表时间：
2005-12-06
影响因子：
11.1
作者：
Grainger, DC;Hurd, D;Busby, SJW
通讯作者：
Busby, SJW
影响因子：
16
作者：
Chen, H;Tang, H;Ebright, RH
通讯作者：
Ebright, RH