Pan-Genome-Wide Identification and Transcriptome-Wide Analysis of DREB Genes That Respond to Biotic and Abiotic Stresses in Cucumber
Pan-Genome-Wide Identification and Transcriptome-Wide Analysis of DREB Genes That Respond to Biotic and Abiotic Stresses in Cucumber
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黄瓜响应生物和非生物胁迫的 DREB 基因的全基因组鉴定和全转录组分析
DOI：
10.3390/agriculture12111879
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发表时间：
2022-11
期刊：
影响因子：
--
通讯作者：
Zhonghai Ren
中科院分区：
文献类型：
--
作者：
Can Wang;Jing Han;Ting Wang;Chunhua Chen;Junyi Liu;Zhixuan Xu;Qingxia Zhang;Lina Wang;Zhonghai Ren
黄瓜（Cucumis sativus L.）的生产经常受到生物和非生物胁迫的损害。尽管脱水反应元件结合（DREB）转录因子在胁迫反应中发挥着重要作用，已在多种植物中得到表征，但人们对黄瓜中DREB基因的全基因组特征及其在不同胁迫下的表达模式知之甚少。在本研究中，我们从 13 个种质的黄瓜泛基因组中鉴定出 55 个 CsDREB，但只有 4 个种质具有所有基因。大多数CsDREB蛋白具有序列长度和/或氨基酸变异，只有其中四种在不同种质之间没有变异。利用‘9930’中的55个CsDREB，我们分析了它们的基因结构、保守结构域、系统发育关系、基因启动子的顺式元件和同线关系，并将它们分为六组。表达模式分析显示，8个CsDREB表现出组成型表达（所有样品中FPKM > 1），并且不同的CsDREB分别在根、茎、叶、卷须、雄花、雌花和子房中表现出特异的高表达，表明这些基因可能对黄瓜的形态建成和发育很重要。此外，总共有 31、22、30 和 9 个 CsDREB 在对热、氯化钠和/或硅、动力霉病和霜霉病处理的响应中分别差异表达。有趣的是，CsDREB33 可以对所有测试的压力做出反应。我们的研究结果为进一步研究DREB基因在黄瓜抗性育种中的功能和机制提供了参考和基础。
The production of cucumber (Cucumis sativus L.) is often harmed by biotic and abiotic stresses. Although the dehydration-responsive element-binding (DREB) transcription factors, playing vital roles in stress responses, have been characterized in several plant species, little is known about the pan-genome characteristics of DREB genes and their expression patterns under different stresses in cucumber. In this study, we identified 55 CsDREBs from the cucumber pan-genomes of 13 accessions, but only four accessions had all the genes. Most of the CsDREB proteins had sequence length and/or amino acid variations, and only four of them had no variation among different accessions. Using the 55 CsDREBs from ‘9930’, we analyzed their gene structures, conserved domains, phylogenetic relationships, gene promoter’s cis-elements and syntenic relationships, and classified them into six groups. Expression pattern analysis revealed that eight CsDREBs showed constitutive expression (FPKM > 1 in all samples), and different CsDREBs showed specifically high expression in root, stem, leaf, tendril, male-flower, female flower, and ovary, respectively, suggesting that these genes might be important for morphogenesis and development in cucumber. Additionally, a total of 31, 22, 30 and nine CsDREBs were differentially expressed in responding to the treatments of heat, NaCl and/or silicon, power mildew and downy mildew, respectively. Interestingly, CsDREB33 could respond to all the tested stresses. Our results provide a reference and basis for further investigation of the function and mechanism of the DREB genes for resistance breeding in cucumber.
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转录组动力学提供了对硅介导的黄瓜植物盐胁迫缓解机制的深入了解
影响因子：
6.8
作者：
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