Trophoblast Major Histocompatibility Complex Class I Expression Is Associated with Immune-Mediated Rejection of Bovine Fetuses Produced by Cloning.
Trophoblast Major Histocompatibility Complex Class I Expression Is Associated with Immune-Mediated Rejection of Bovine Fetuses Produced by Cloning.
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DOI：
10.1095/biolreprod.115.136523
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发表时间：
2016-08
影响因子：
3.6
通讯作者：
Davies CJ
中科院分区：
文献类型：
--
作者：
Rutigliano HM;Thomas AJ;Wilhelm A;Sessions BR;Hicks BA;Schlafer DH;White KL;Davies CJ
滋养层细胞从牛体细胞核移植（SCNT）概念表达主要组织相容性复合物I类（MHC-I）蛋白在妊娠早期，这可能是显着的头三个月的胚胎死亡率在这些怀孕的原因之一。建立了MHC-I同源相容（n = 9）、同源不相容（n = 8）和异源不相容（n = 5）SCNT妊娠。对照组由8例人工授精妊娠组成。在妊娠第35 ± 1天采集子宫和胎盘样本，通过免疫组织化学方法检测MHC-I、白细胞标志物和细胞因子的表达。所有SCNT妊娠的滋养层细胞均表达MHC-I，而年龄匹配的对照妊娠的滋养层细胞MHC-I表达阴性。SCNT妊娠滋养层细胞MHC-I抗原表达与子宫内膜淋巴细胞浸润相关。此外，MHC-I-不相容的孕体，特别是异源性不相容的，诱导更明显的淋巴细胞浸润比MHC-I-兼容的孕体。与对照组相比，SCNT妊娠子宫内膜中表达分化簇（CD）3、γ/δ TCR和MHC-II的细胞增加。与MHC-I相容和对照妊娠相比，MHC-I不相容妊娠的CD 4+淋巴细胞增加。与纯合子SCNT和对照妊娠相比，MHC-I杂合子不相容的SCNT妊娠中CD 8+、FOXP 3+和自然杀伤细胞增加。
Trophoblast cells from bovine somatic cell nuclear transfer (SCNT) conceptuses express major histocompatibility complex class I (MHC-I) proteins early in gestation, and this may be one cause of the significant first-trimester embryonic mortality observed in these pregnancies. MHC-I homozygous-compatible (n = 9), homozygous-incompatible (n = 8), and heterozygous-incompatible (n = 5) SCNT pregnancies were established. The control group consisted of eight pregnancies produced by artificial insemination. Uterine and placental samples were collected on Day 35 ± 1 of pregnancy, and expression of MHC-I, leukocyte markers, and cytokines were examined by immunohistochemistry. Trophoblast cells from all SCNT pregnancies expressed MHC-I, while trophoblast cells from age-matched control pregnancies were negative for MHC-I expression. Expression of MHC-I antigens by trophoblast cells from SCNT pregnancies was associated with lymphocytic infiltration in the endometrium. Furthermore, MHC-I-incompatible conceptuses, particularly the heterozygous-incompatible ones, induced a more pronounced lymphocytic infiltration than MHC-I-compatible conceptuses. Cells expressing cluster of differentiation (CD) 3, gamma/deltaTCR, and MHC-II were increased in the endometrium of SCNT pregnancies compared to the control group. CD4+ lymphocytes were increased in MHC-I-incompatible pregnancies compared to MHC-I-compatible and control pregnancies. CD8+, FOXP3+, and natural killer cells were increased in MHC-I heterozygous-incompatible SCNT pregnancies compared to homozygous SCNT and control pregnancies.
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DOI：
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发表时间：
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影响因子：
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影响因子：
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