Bacillus Calmette-Guérin Suppresses Asthmatic Responses via CD4(+)CD25(+) Regulatory T Cells and Dendritic Cells.
Bacillus Calmette-Guérin Suppresses Asthmatic Responses via CD4(+)CD25(+) Regulatory T Cells and Dendritic Cells.
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DOI：
10.4168/aair.2014.6.3.201
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发表时间：
2014-05
期刊：
影响因子：
--
通讯作者：
Hong SJ
中科院分区：
文献类型：
--
作者：
Kim YJ;Kim HJ;Kang MJ;Yu HS;Seo JH;Kim HY;Park SJ;Lee YC;Hong SJ
已知卡介苗（BCG）在哮喘小鼠模型中抑制哮喘反应并诱导树突状细胞（DC）成熟。成熟的DC在调节性T细胞（Treg）的分化中起关键作用，已知Treg调节过敏性炎症反应。为了研究BCG是否以DCs介导的方式调节TcR，我们在哮喘小鼠模型中进行了分析。BALB/c小鼠腹腔注射BCG或静脉注射BCG刺激的DC，然后用卵清蛋白（OVA）致敏和激发。分析小鼠的支气管高反应性（BHR）、支气管肺泡灌洗液（BAL）中炎性细胞的流入以及肺的组织病理学变化。为了确定机制，分析血清中的IgE、IgG 1和IgG 2a，并且用抗CD 25单克隆抗体（mAb）耗尽小鼠中的CD 25 + T细胞。BCG和BCG刺激的DC的转移都抑制了哮喘反应的所有方面，即BHR、总IgE和OVA特异性IgE和IgG的产生以及肺嗜酸性粒细胞炎症。抗CD 25 mAb治疗逆转了这些作用。BCG可通过DC介导的Tregs相关机制减轻哮喘小鼠模型的过敏性炎症。
Bacillus Calmette-Guérin (BCG) is known to suppress the asthmatic responses in a murine model of asthma and to induce dendritic cells (DCs) maturation. Mature DCs play a crucial role in the differentiation of regulatory T cells (Tregs), which are known to regulate allergic inflammatory responses. To investigate whether BCG regulates Tregs in a DCs-mediated manner, we analyzed in a murine model of asthma. BALB/c mice were injected intraperitoneally with BCG or intravenously with BCG-stimulated DCs and then sensitized and challenged with ovalbumin (OVA). Mice were analysed for bronchial hyperresponsiveness (BHR), the influx of inflammatory cells in the bronchoalveolar lavage (BAL) fluid, and histopathological changes in the lung. To identify the mechanisms, IgE, IgG1 and IgG2a in the serum were analysed and the CD25+ Tregs in the mice were depleted with anti-CD25 monoclonal antibody (mAb). BCG and the transfer of BCG-stimulated DCs both suppressed all aspects of the asthmatic responses, namely, BHR, the production of total IgE and OVA-specific IgE and IgGs, and pulmonary eosinophilic inflammation. Anti-CD25mAb treatment reversed these effects. BCG can attenuate the allergic inflammation in a mouse model of asthma by a Tregs-related mechanism that is mediated by DCs.
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DOI：
10.4168/aair.2011.3.4.236
发表时间：
2011-10
期刊：
Allergy, asthma & immunology research
影响因子：
--
作者：
Lee SH;Park JS;Park CS
通讯作者：
Park CS
DOI：
10.1165/rcmb.2009-0040oc
发表时间：
2010-08-01
影响因子：
6.4
作者：
Christ, Ana P.;Rodriguez, Dunia;Russo, Momtchilo
通讯作者：
Russo, Momtchilo
影响因子：
64.5
作者：
Geijtenbeek, TBH;Torensma, R;Figdor, CG
通讯作者：
Figdor, CG
影响因子：
8.2
作者：
Li, Qian;Shen, Hua-hao
通讯作者：
Shen, Hua-hao
影响因子：
4.4
作者：
Han, Yanmei;Guo, Qiuli;Cao, Xuetao
通讯作者：
Cao, Xuetao