P-selectin and P-selectin glycoprotein ligand 1 are major determinants for Th1 cell recruitment to nonlymphoid effector sites in the intestinal lamina propria.
P-selectin and P-selectin glycoprotein ligand 1 are major determinants for Th1 cell recruitment to nonlymphoid effector sites in the intestinal lamina propria.
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DOI：
10.1084/jem.20020691
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发表时间：
2003-08-04
期刊：
影响因子：
--
通讯作者：
Barrett TA
中科院分区：
文献类型：
--
作者：
Haddad W;Cooper CJ;Zhang Z;Brown JB;Zhu Y;Issekutz A;Fuss I;Lee HO;Kansas GS;Barrett TA
激活的T细胞亚群向效应器免疫反应部位的募集是由次级淋巴组织中激活后诱导的归巢受体介导的。采用过继转移模型，检测了在完全弗氏佐剂中，经腹腔抗原激活的CD4+T细胞在肠道内的募集。结果表明，活化的CD4+T细胞聚集到肠道Peyer‘s结(PP)和固有层(LP)，上调功能性P-选择素糖蛋白配体1(PSGL-1)的表达。阻断IL-12可抑制PSGL-1的功能表达，并使PP和LP的CD4+T细胞募集减少40%。P-选择素阻断可使激活细胞的LP募集减少56%，而不影响PP募集。过继转移分化的T细胞亚群后3d的研究表明，Th1细胞被招募到小肠PP和LP，而不是Th2细胞。粘膜寻址细胞黏附分子阻断使Th1向PP和LP的募集分别减少90%和72%，而P-选择素阻断则使PP和LP的Th1募集分别减少18%和84%。这些数据表明，IL-12诱导的功能性PSGL-1表达是Th1效应细胞募集到非炎症性和炎症性肠道的主要决定因素。
The recruitment of activated T cell subsets to sites of effector immune responses is mediated by homing receptors induced upon activation in secondary lymphoid tissue. Using an adoptive transfer model, the intestinal recruitment of CD4+ T cells activated with intraperitoneal antigen in complete Freund's adjuvant was examined. The data demonstrate that activated CD4+ T cells recruited to intestinal Peyer's patches (PP) and lamina propria (LP) up-regulate functional P-selectin glycoprotein ligand 1 (PSGL-1). Blockade of IL-12 inhibited functional PSGL-1 expression and reduced PP and LP CD4+ T cell recruitment by >40%. P-Selectin blockade reduced LP recruitment of activated cells by 56% without affecting PP recruitment. Studies of mice examined 3 d after adoptive transfer of differentiated T cell subsets revealed that Th1 but not Th2 cells were recruited to small intestine PP and LP. Mucosal addressin cell adhesion molecule blockade reduced Th1 recruitment to PP by 90% and to LP by >72%, whereas P-selectin blockade reduced Th1 recruitment to PP by 18% and Th1 recruitment to LP by 84%. These data suggest that IL-12–induced functional PSGL-1 expression is a major determinant for the recruitment of Th1 effector cells to noninflamed as well as inflamed intestine.
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DOI：
10.1084/jem.20011502
发表时间：
2002-01-07
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Campbell DJ;Butcher EC
通讯作者：
Butcher EC
影响因子：
64.8
作者：
Austrup, F;Vestweber, D;Hamann, A
通讯作者：
Hamann, A
DOI：
10.1084/jem.193.8.987
发表时间：
2001-04-16
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Iezzi G;Scheidegger D;Lanzavecchia A
通讯作者：
Lanzavecchia A
DOI：
10.1084/jem.188.12.2225
发表时间：
1998-12-21
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Wagers AJ;Waters CM;Stoolman LM;Kansas GS
通讯作者：
Kansas GS
影响因子：
4.4
作者：
Lim, YC;Xie, HJ;Luscinskas, FW
通讯作者：
Luscinskas, FW