The immediate upstream region of the 5'-UTR from the AUG start codon has a pronounced effect on the translational efficiency in Arabidopsis thaliana.
The immediate upstream region of the 5'-UTR from the AUG start codon has a pronounced effect on the translational efficiency in Arabidopsis thaliana.
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DOI：
10.1093/nar/gkt864
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发表时间：
2014-01
影响因子：
14.9
通讯作者：
Hwang I
中科院分区：
文献类型：
--
作者：
Kim Y;Lee G;Jeon E;Sohn EJ;Lee Y;Kang H;Lee DW;Kim DH;Hwang I
翻译起始位点周围的核苷酸序列是转录后调控的重要顺式作用元件。然而，尚未完全理解5′-非翻译区（5′-UTR）的序列背景如何影响单个mRNA的翻译效率。在这项研究中，我们提供的证据表明，在拟南芥基因的5′-UTR的核苷酸序列显示出很大的差异，在翻译效率有很大的差异，超过200倍。在这四种类型的核苷酸中，A残基是拟南芥基因5′-UTR的-1至-21位最有利的核苷酸。特别是，5′-UTR中-1至-5位的A残基是高水平翻译效率所必需的。相比之下，5′-UTR中-1至-5位的T残基是翻译效率最差的核苷酸。此外，在不同的植物物种中，5′-UTR的-1到-21区域的序列背景的影响是保守的。基于这些观察，我们提出，序列上下文紧接上游的AUG起始密码子起着至关重要的作用，在确定植物基因的翻译效率。
The nucleotide sequence around the translational initiation site is an important cis-acting element for post-transcriptional regulation. However, it has not been fully understood how the sequence context at the 5′-untranslated region (5′-UTR) affects the translational efficiency of individual mRNAs. In this study, we provide evidence that the 5′-UTRs of Arabidopsis genes showing a great difference in the nucleotide sequence vary greatly in translational efficiency with more than a 200-fold difference. Of the four types of nucleotides, the A residue was the most favourable nucleotide from positions −1 to −21 of the 5′-UTRs in Arabidopsis genes. In particular, the A residue in the 5′-UTR from positions −1 to −5 was required for a high-level translational efficiency. In contrast, the T residue in the 5′-UTR from positions −1 to −5 was the least favourable nucleotide in translational efficiency. Furthermore, the effect of the sequence context in the −1 to −21 region of the 5′-UTR was conserved in different plant species. Based on these observations, we propose that the sequence context immediately upstream of the AUG initiation codon plays a crucial role in determining the translational efficiency of plant genes.
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