The Arabidopsis alkaline ceramidase TOD1 is a key turgor pressure regulator in plant cells.
The Arabidopsis alkaline ceramidase TOD1 is a key turgor pressure regulator in plant cells.
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拟南芥碱性神经酰胺酶 TOD1 是植物细胞中关键的膨压调节剂
DOI：
10.1038/ncomms7030
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发表时间：
2015-01-16
影响因子：
16.6
通讯作者：
Yang, Wei-Cai
中科院分区：
文献类型：
--
作者：
Chen, Li-Yu;Shi, Dong-Qiao;Zhang, Wen-Juan;Tang, Zuo-Shun;Liu, Jie;Yang, Wei-Cai
膨压在构成植物和真菌的有壁细胞的生长和运动中起着关键作用。然而，调节膨压的分子机制以及膨压和细胞壁重塑之间的协调对细胞生长仍然知之甚少。在这里，我们报告的表征拟南芥TurgOr调节缺陷1（TOD 1），这是优先表达在花粉管和角果保卫细胞。我们证明，TOD 1是高尔基体定位的碱性神经酰胺酶。tod 1突变体花粉管比野生型有更高的膨压力，并且在雌蕊和琼脂糖培养基中均表现出生长迟缓。此外，tod 1保卫细胞是不敏感的脱落酸（阿坝）诱导的气孔关闭，而鞘氨醇-1-磷酸，一个假定的下游组件的阿坝信号和碱性神经酰胺酶的产品，促进关闭在野生型和tod 1。我们的数据表明，TOD 1的行为在膨压调节保卫细胞和花粉管。
Turgor pressure plays pivotal roles in the growth and movement of walled cells that make up plants and fungi. However, the molecular mechanisms regulating turgor pressure and the coordination between turgor pressure and cell wall remodelling for cell growth remain poorly understood. Here, we report the characterization of Arabidopsis TurgOr regulation Defect 1 (TOD1), which is preferentially expressed in pollen tubes and silique guard cells. We demonstrate that TOD1 is a Golgi-localized alkaline ceramidase. tod1 mutant pollen tubes have higher turgor than wild type and show growth retardation both in pistils and in agarose medium. In addition, tod1 guard cells are insensitive to abscisic acid (ABA)-induced stomatal closure, whereas sphingosine-1-phosphate, a putative downstream component of ABA signalling and product of alkaline ceramidases, promotes closure in both wild type and tod1. Our data suggest that TOD1 acts in turgor pressure regulation in both guard cells and pollen tubes.
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影响因子：
64.8
作者：
ISHIJIMA, A;DOI, T;YANAGIDA, T
通讯作者：
YANAGIDA, T
影响因子：
14.8
作者：
Gietz, R. Daniel;Schiestl, Robert H.
通讯作者：
Schiestl, Robert H.
影响因子：
13.8
作者：
Combet, C;Blanchet, C;Deléage, G
通讯作者：
Deléage, G
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
--
作者：
ALEXANDER, MP
通讯作者：
ALEXANDER, MP