YwdL in Bacillus cereus: its role in germination and exosporium structure.
YwdL in Bacillus cereus: its role in germination and exosporium structure.
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DOI：
10.1371/journal.pone.0023801
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Bullough PA
中科院分区：
文献类型：
--
作者：
Terry C;Shepherd A;Radford DS;Moir A;Bullough PA
在蜡样芽孢杆菌组的成员中，孢子的最外层是外孢壁，它与宿主和环境相互作用。人们已经努力鉴定孢子外膜的蛋白质，但迄今为止只有少数蛋白质被表征，并且它们在确定孢子结构和孢子功能中的作用仍然知之甚少。我们已经表征了外孢子蛋白 YwdL。 ΔywdL 孢子有一个更脆弱的外壁，在反复冻融时会受到损坏，尽管没有证据表明抵抗力特性发生了改变，而且外衣看起来完好无损。免疫金标记和抗 YwdL 抗体的蛋白质印迹鉴定出 YwdL 仅位于蜡样芽孢杆菌和苏云金芽孢杆菌的外孢子内表面。我们得出的结论是，YwdL 对于形成坚固的外孢壁很重要，但不需要维持基底层内的晶体组装或毛状绒毛结构的附着。 ΔywdL 孢子无法响应 CaDPA 萌发，并且改变了萌发特性，这一表型证实了皮层裂解酶 CwlJ 在外壳中定位的预期缺陷。
In members of the Bacillus cereus group the outermost layer of the spore is the exosporium, which interacts with hosts and the environment. Efforts have been made to identify proteins of the exosporium but only a few have so far been characterised and their role in determining spore architecture and spore function is still poorly understood. We have characterised the exosporium protein, YwdL. ΔywdL spores have a more fragile exosporium, subject to damage on repeated freeze-thawing, although there is no evidence of altered resistance properties, and coats appear intact. Immunogold labelling and Western blotting with anti-YwdL antibodies identified YwdL to be located exclusively on the inner surface of the exosporium of B. cereus and B. thuringiensis. We conclude that YwdL is important for formation of a robust exosporium but is not required to maintain the crystalline assembly within the basal layer or for attachment of the hairy nap structure. ΔywdL spores are unable to germinate in response to CaDPA, and have altered germination properties, a phenotype that confirms the expected defect in localization of the cortex lytic enzyme CwlJ in the coat.
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