Projection neurons from the central nucleus of the amygdala to the nucleus pontis oralis.
Projection neurons from the central nucleus of the amygdala to the nucleus pontis oralis.
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DOI：
10.1002/jnr.22554
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发表时间：
2011-03
影响因子：
4.2
通讯作者：
Chase, Michael H.
中科院分区：
文献类型：
--
作者：
Fung, Simon J.;Xi, MingChu;Zhang, JianHua;Torterolo, Pablo;Sampogna, Sharon;Morales, Francisco R.;Chase, Michael H.
本逆行标记研究的目的是确定的存在和模式的预测从中央核杏仁核（CNA）的个别分区的脑桥口核（NPO），这是一个关键的脑干网站参与的生成和维持的主动（REM）睡眠。用逆行示踪剂霍乱毒素B亚基（CTB）标记CNA的投射，通过微离子电渗法将其单侧注射到NPO中。对杏仁核切片进行免疫染色，以鉴定CTB标记的CNA神经元和含有CTB加上囊泡谷氨酸转运蛋白2（VGLUT 2）的CNA神经元，VGLUT 2是谷氨酸能神经元的标志物。组织学分析显示，逆行标记的神经元，项目的NPO本地化，同侧，内侧，外侧和囊内的CNA细分。此外，所有逆行标记的CNA神经元的相当大的比例（24%）也表现出VGLUT 2免疫反应性。本研究表明，海马能神经元，这是目前在各种细分的CNA，项目直接向NPO。这些数据为以下假设提供了证据：参与主动睡眠控制的NPO神经元是由杏仁核的突触能投射激活的。
The present retrograde labeling study was designed to determine the presence and pattern of projections from individual subdivisions of the central nucleus of the amygdala (CNA) to the nucleus pontis oralis (NPO), which is a critical brainstem site involved for the generation and maintenance of active (REM) sleep. Projections from the CNA were labeled with the retrograde tracer, cholera toxin B-subunit (CTB), which was injected, unilaterally, via microiontophoresis, into the NPO. Sections of the amygdala were immunostained in order to identify CTB-labeled CNA neurons and CNA neurons that contained CTB plus the vesicular glutamate transporter 2 (VGLUT2), which is a marker for glutamatergic neurons. Histological analyses revealed that retrogradely-labeled neurons that project to the NPO were localized, ipsilaterally, within the medial, lateral and capsular subdivisions of the CNA. In addition, a substantial proportion (24%) of all retrogradely-labeled CNA neurons also exhibited VGLUT2 immunoreactivity. The present study demonstrates that glutamatergic neurons, which are present within various subdivisions of the CNA, project directly to the NPO. These data lend credence to the hypothesis that NPO neurons that are involved in the control of active sleep are activated by glutamatergic projections from the amygdala.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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