Long-Term Maintenance of Acinar Cells in Human Submandibular Glands After Radiation Therapy.
Long-Term Maintenance of Acinar Cells in Human Submandibular Glands After Radiation Therapy.
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DOI：
10.1016/j.ijrobp.2020.10.037
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发表时间：
2021-03-15
期刊：
影响因子：
--
通讯作者：
Ovitt CE
中科院分区：
文献类型：
--
作者：
Luitje ME;Israel AK;Cummings MA;Giampoli EJ;Allen PD;Newlands SD;Ovitt CE
在一项回顾性与前瞻性相结合的研究中，对接受头颈部癌症放射治疗后的人类唾液腺进行了研究。目的是评估放射治疗后腺泡细胞的损失和形态变化，并确定受照射的唾液腺是否具有再生潜力。
在放射治疗（RT）完成后的不同时间间隔，从16名患者身上收集了受照射的人类下颌下腺和腮腺。从14名未接受放射治疗的患者身上收集了对照样本。使用免疫组织化学对组织切片进行分析，以对分子标记物进行染色。
在RT后不到1年分离出的人类下颌下腺和腮腺显示腺泡细胞几乎完全丧失。然而，在RT后较晚时间间隔分离出的所有样本中都观察到了表达功能性分泌标记物的腺泡单位。与仅接受RT治疗的腺体相比，接受放疗和化疗联合治疗的腺体中腺泡细胞数量明显减少，纤维化增加。与未受照射的对照样本相比，受照射的样本显示导管细胞角蛋白标记物的染色增加，以及许多细胞共同表达腺泡细胞和导管细胞特异性标记物。
RT后，人类下颌下腺中的腺泡细胞簇可维持数年。存活的腺泡细胞保留增殖潜力，尽管没有发生明显的再生。持续的DNA损伤、增加的纤维化和改变的细胞特性提示了可能损害再生的机制。
In a combined retrospective and prospective study, human salivary glands were investigated after radiation treatment for head and neck cancers. The aim was to assess acinar cell loss and morphologic changes after radiation therapy and to determine whether irradiated salivary glands have regenerative potential. Irradiated human submandibular and parotid salivary glands were collected from 16 patients at a range of time intervals after completion of radiation therapy (RT). Control samples were collected from 14 patients who had not received radiation treatments. Tissue sections were analyzed using immunohistochemistry to stain for molecular markers. Human submandibular and parotid glands isolated less than 1 year after RT showed a near complete loss of acinar cells. However, acinar units expressing functional secretory markers were observed in all samples isolated at later intervals after RT. Significantly lower acinar cell numbers and increased fibrosis were found in glands treated with combined radiation and chemotherapy, in comparison to glands treated with RT alone. Irradiated samples showed increased staining for duct cell keratin markers, as well as many cells coexpressing acinar- and duct cell–specific markers, in comparison to nonirradiated control samples. After RT, acinar cell clusters are maintained in human submandibular glands for years. The surviving acinar cells retain proliferative potential, although significant regeneration does not occur. Persistent DNA damage, increased fibrosis, and altered cell identity suggest mechanisms that may impair regeneration.
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影响因子：
10.5
作者：
Lo HG;Jin RU;Sibbel G;Liu D;Karki A;Joens MS;Madison BB;Zhang B;Blanc V;Fitzpatrick JA;Davidson NO;Konieczny SF;Mills JC
通讯作者：
Mills JC
影响因子：
3.5
作者：
Ingalls, Matthew H.;Hollomon, Andrew J.;Ovitt, Catherine E.
通讯作者：
Ovitt, Catherine E.
DOI：
10.1007/bf00713290
发表时间：
1987-01-01
期刊：
VIRCHOWS ARCHIV A-PATHOLOGICAL ANATOMY AND HISTOPATHOLOGY
影响因子：
--
作者：
BORN, IA;SCHWECHHEIMER, K;OTTO, HF
通讯作者：
OTTO, HF
影响因子：
3.2
作者：
Nam, Kihoon;Maruyama, Christina L.;Baker, Olga J.
通讯作者：
Baker, Olga J.
DOI：
10.1016/s0360-3016(01)01717-5
发表时间：
2001-11-15
影响因子：
7
作者：
Roesink, JM;Moerland, MA;Terhaard, CHJ
通讯作者：
Terhaard, CHJ