Gliotactin, a novel marker of tricellular junctions, is necessary for septate junction development in Drosophila.
Gliotactin, a novel marker of tricellular junctions, is necessary for septate junction development in Drosophila.
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DOI：
10.1083/jcb.200303192
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发表时间：
2003-06-09
期刊：
影响因子：
--
通讯作者：
Auld VJ
中科院分区：
文献类型：
--
作者：
Schulte J;Tepass U;Auld VJ
与紧密连接类似，隔连接（SJs）起着跨上皮渗透屏障的作用。Gliotactin（Gli）是一种胆碱酯酶样分子，对血神经屏障的完整性是必需的，因此可能有助于SJ的发育或功能。为了解决这一假设，我们分析了Gli表达和Gli突变体表型在果蝇上皮细胞。在Gli突变体中，SJ标记neurexin-IV、大圆盘和小珊瑚的定位被破坏。此外，SJ屏障功能丧失，如通过染料渗透性测定所确定的。这些数据表明，Gli是必要的SJ形成。令人惊讶的是，Gli分布只与其他SJ标记物共定位在三细胞连接处，这表明Gli在SJ发育中具有独特的功能。Gli突变体的超微结构分析支持这一观点。与其他SJ突变体中隔膜缺失相反，Gli突变体中存在隔膜，但接头具有不成熟的形态。我们提出了一个模型，即Gli的行为在三细胞交界处绑定，锚，或紧凑SJ股顶端SJ发展过程中。
Septate junctions (SJs), similar to tight junctions, function as transepithelial permeability barriers. Gliotactin (Gli) is a cholinesterase-like molecule that is necessary for blood–nerve barrier integrity, and may, therefore, contribute to SJ development or function. To address this hypothesis, we analyzed Gli expression and the Gli mutant phenotype in Drosophila epithelia. In Gli mutants, localization of SJ markers neurexin-IV, discs large, and coracle are disrupted. Furthermore, SJ barrier function is lost as determined by dye permeability assays. These data suggest that Gli is necessary for SJ formation. Surprisingly, Gli distribution only colocalizes with other SJ markers at tricellular junctions, suggesting that Gli has a unique function in SJ development. Ultrastructural analysis of Gli mutants supports this notion. In contrast to other SJ mutants in which septa are missing, septa are present in Gli mutants, but the junction has an immature morphology. We propose a model, whereby Gli acts at tricellular junctions to bind, anchor, or compact SJ strands apically during SJ development.
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单个基因产物Claudin -1或-2，在成纤维细胞中重构紧密结合的连接链和新兵。
影响因子：
7.8
作者：
Furuse, M;Sasaki, H;Fujimoto, K;Tsukita, S
通讯作者：
Tsukita, S
紧密连接链内和之间异质claudin物种相互作用的方式。
影响因子：
7.8
作者：
Furuse, M;Sasaki, H;Tsukita, S
通讯作者：
Tsukita, S
影响因子：
16.2
作者：
Leiserson, WM;Harkins, EW;Keshishian, H
通讯作者：
Keshishian, H
DOI：
10.1093/protein/11.6.415
发表时间：
1998-06-01
期刊：
PROTEIN ENGINEERING
影响因子：
--
作者：
Botti, SA;Felder, CE;Silman, I
通讯作者：
Silman, I
影响因子：
7.8
作者：
FRISTROM, DK
通讯作者：
FRISTROM, DK