Rapid and sensitive detection of human astrovirus in water samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye.
Rapid and sensitive detection of human astrovirus in water samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye.
复制标题
使用羟基萘酚蓝染料进行环介导等温扩增，快速灵敏地检测水样中的人星状病毒
DOI：
10.1186/1471-2180-14-38
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发表时间：
2014-02-14
期刊：
影响因子：
4.2
通讯作者：
Wang ZJ
中科院分区：
文献类型：
--
作者：
Yang BY;Liu XL;Wei YM;Wang JQ;He XQ;Jin Y;Wang ZJ
目的建立一种快速、灵敏、廉价的逆转录环介导等温扩增(RT-LAMP)方法检测星状病毒。结果LAMP用于体外RNA转录本的检测下限为3.6×10拷贝·μL-1，与目前使用的聚合酶链式反应方法一样灵敏。然而，用羟基萘酚蓝染料(HNB)染色后，肉眼可以鉴定出不同颜色的灯产品。未观察到与其他胃肠道病毒(轮状病毒和诺如病毒)的交叉反应，表明LAMP具有较高的特异性。结论应用RT-LAMP技术检测再生水样中的星状病毒是一种廉价、灵敏、特异、快速的方法。RT-LAMP方法可以简便地用于星状病毒的特异性检测，并有可能作为现场筛选试验使用。
BackgroundThe aim of this paper was to develop a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid, sensitive and inexpensive detection of astrovirus.ResultsThe detection limit of LAMP usingin vitroRNA transcripts was 3.6×10 copies·μL-1, which is as sensitive as the presently used PCR assays. However, the LAMP products could be identified as different colors with the naked eye following staining with hydroxynaphthol blue dye (HNB). No cross-reactivity with other gastroenteric viruses (rotavirus and norovirus) was observed, indicating the relatively high specificity of LAMP. The RT-LAMP method with HNB was used to effectively detect astrovirus in reclaimed water samples.ConclusionsThe LAMP technique described in this study is a cheap, sensitive, specific and rapid method for the detection of astrovirus. The RT-LAMP method can be simply applied for the specific detection of astrovirus and has the potential to be utilized in the field as a screening test.
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影响因子：
12.7
作者：
Guo, Li;Xu, Xiwei;Hung, Tao
通讯作者：
Hung, Tao
影响因子：
3.1
作者：
Ma, Xue-jun;Shu, Yue-long;Dong, Xiao-ping
通讯作者：
Dong, Xiao-ping
影响因子：
4.4
作者：
Katayama, H;Shimasaki, A;Ohgaki, S
通讯作者：
Ohgaki, S
影响因子：
4
作者：
Mao, X. L.;Zhou, S.;Qin, Q. W.
通讯作者：
Qin, Q. W.
DOI：
10.1080/10934520701795731
发表时间：
2008-01-01
影响因子：
2.1
作者：
He, Xiao Q.;Cheng, Li;Wang, Zi J.
通讯作者：
Wang, Zi J.