Controlled Adipose-derived Stromal Cells Differentiation into Adipose and Endothelial Cells in a 3D Structure Established by Cell-assembly Technique
Controlled Adipose-derived Stromal Cells Differentiation into Adipose and Endothelial Cells in a 3D Structure Established by Cell-assembly Technique
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通过细胞组装技术建立 3D 结构，控制脂肪源性基质细胞分化为脂肪和内皮细胞
DOI：
10.1177/0883911509102794
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发表时间：
2009-05
影响因子：
1.7
通讯作者：
Yao, Rui
中科院分区：
文献类型：
--
作者：
Xu, Mingen;Yan, Yongnian;Liu, Haixia;Wang, Xiaohong;Yao, Rui
在体外使组织血管化的同时工程化厚且复杂的组织的主要障碍之一是在组织生长和结构组织期间维持细胞活力。利用脂肪基质细胞（ADS），通过细胞组装技术建立多细胞系统。尝试根据ADS细胞在有序3D结构内的位置来控制ADS细胞分化成不同的靶细胞类型。油红0染色证实结构中的ADS细胞分化为具有球形形状的脂肪细胞，而免疫染色测试证实内皮生长因子诱导通道壁上的ADS细胞分化为成熟的内皮细胞，然后在整个工程化的3D结构中组织成管状结构。内皮细胞释放内皮素-1和一氧化氮的规律与体内情况一致。本研究为体外构建有序的内皮血管网络提供了新的途径，在脂肪组织工程中具有潜在的应用价值。
One of the major obstacles in engineering thick and complex tissues while vascularizing tissues in vitro is to maintain cell viability during tissue growth and structural organization. Adipose-derived stromal (ADS) cells were used to establish a multicellular system through a cell-assembly technique. Attempts were made to control ADS cells differentiation into different targeted cell types according to their positions within an orderly 3D structure. Oil red 0 staining confirmed that the ADS cells in the structure differentiated into adipocytes with a spherical shape while immunostaining tests confirmed that the endothelial growth factor induced ADS cells on the walls of channels differentiated into mature endothelial cells and then organized into tubular structures throughout the engineered 3D structure. The endothelin-1 and nitric oxide release rules of the endothelial cells were coincidental with those in vivo. This study provides a new approach to engineer orderly endothelial vessel networks in vitro and has potential applications in adipose-tissue engineering.
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影响因子：
3.3
作者：
Zuk, PA;Zhu, M;Hedrick, MH
通讯作者：
Hedrick, MH
影响因子：
1.7
作者：
Wei Xu;X. Wang;Yongnian Yan;Weizhen Zheng;Z. Xiong;F. Lin;R. Wu;Renji Zhang
通讯作者：
Wei Xu;X. Wang;Yongnian Yan;Weizhen Zheng;Z. Xiong;F. Lin;R. Wu;Renji Zhang
影响因子：
14
作者：
von Heimburg, D;Zachariah, S;Pallua, N
通讯作者：
Pallua, N
影响因子：
1.7
作者：
Cui, Tongkui;Wang, Xiaohong;Zhang, Renji
通讯作者：
Zhang, Renji
影响因子：
3
作者：
Cui, Tongkui;Yan, Yongnian;Wang, Xiaohong
通讯作者：
Wang, Xiaohong