VGLUT1 or VGLUT2 mRNA-positive neurons in spinal trigeminal nucleus provide collateral projections to both the thalamus and the parabrachial nucleus in rats.
VGLUT1 or VGLUT2 mRNA-positive neurons in spinal trigeminal nucleus provide collateral projections to both the thalamus and the parabrachial nucleus in rats.
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三叉神经脊髓核中的 VGLUT1 或 VGLUT2 mRNA 阳性神经元为大鼠的丘脑和臂旁核提供侧支投射
DOI：
10.1186/s13041-018-0362-y
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发表时间：
2018-04-12
期刊：
影响因子：
3.6
通讯作者：
Li JL
中科院分区：
文献类型：
--
作者：
Zhang CK;Li ZH;Qiao Y;Zhang T;Lu YC;Chen T;Dong YL;Li YQ;Li JL
三叉神经-丘脑(T-T)通路和三叉神经-臂旁神经(T-P)通路分别与口面部疼痛的感觉辨别和情感/情绪方面密切相关。这些T-T和T-P投射纤维来自三叉神经脊束核(VSP)。我们先前发现，在成年大鼠的VSP内分布有许多囊泡型谷氨酸转运体(VGLUT1和/或VGLUT2)mRNA阳性神经元，这些神经元大多将轴突投射到丘脑或小脑。然而，是否存在向丘脑和臂旁核(PBN)发送轴突的VGLUT1或VGLUT2基因阳性投射神经元尚未见报道。因此，在本研究中，我们使用双重逆行追踪结合VGLUT1或VGLUT2 mRNA的荧光原位杂交(FISH)来鉴定向丘脑和PBN发送侧支投射的VGLUT1或VGLUT2 mRNA神经元的存在。VSP内向丘脑和PBN发出侧支投射的神经元主要为VGLUT2 mRNA阳性神经元，在VSP的口(Vo)、极间(Vi)和尾侧(Vc)亚核中分别占90.3%、93.0%和85.4%。此外，在唇部注射福尔马林后，约34.0%的Vc侧支投射神经元呈Fos免疫阳性，部分降钙素基因相关肽(CGRP)免疫阳性轴索静脉曲张与Vc侧支投射神经元直接接触。这些结果表明，VSP内的大多数侧支投射神经元，特别是Vc内的侧支投射神经元，主要表达VGLUT2，可能通过轴突将伤害性信息直接传递到丘脑和PBN。
The trigemino-thalamic (T-T) and trigemino-parabrachial (T-P) pathways are strongly implicated in the sensory-discriminative and affective/emotional aspects of orofacial pain, respectively. These T-T and T-P projection fibers originate from the spinal trigeminal nucleus (Vsp). We previously determined that many vesicular glutamate transporter (VGLUT1 and/or VGLUT2) mRNA-positive neurons were distributed in the Vsp of the adult rat, and most of these neurons sent their axons to the thalamus or cerebellum. However, whether VGLUT1 or VGLUT2 mRNA-positive projection neurons exist that send their axons to both the thalamus and the parabrachial nucleus (PBN) has not been reported. Thus, in the present study, dual retrograde tract tracing was used in combination with fluorescence in situ hybridization (FISH) for VGLUT1 or VGLUT2 mRNA to identify the existence of VGLUT1 or VGLUT2 mRNA neurons that send collateral projections to both the thalamus and the PBN. Neurons in the Vsp that send collateral projections to both the thalamus and the PBN were mainly VGLUT2 mRNA-positive, with a proportion of 90.3%, 93.0% and 85.4% in the oral (Vo), interpolar (Vi) and caudal (Vc) subnucleus of the Vsp, respectively. Moreover, approximately 34.0% of the collateral projection neurons in the Vc showed Fos immunopositivity after injection of formalin into the lip, and parts of calcitonin gene-related peptide (CGRP)-immunopositive axonal varicosities were in direct contact with the Vc collateral projection neurons. These results indicate that most collateral projection neurons in the Vsp, particularly in the Vc, which express mainly VGLUT2, may relay orofacial nociceptive information directly to the thalamus and PBN via axon collaterals.
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