Temporospatial expression and cellular localization of VGLUT3 in the rat cochlea
Temporospatial expression and cellular localization of VGLUT3 in the rat cochlea
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大鼠耳蜗中 VGLUT3 的时空表达和细胞定位
DOI：
10.1016/j.brainres.2013.09.019
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发表时间：
2013-11
期刊：
影响因子：
2.9
通讯作者：
Gong SS
中科院分区：
文献类型：
--
作者：
Peng Z;Wang GP;Zeng R;Guo JY;Chen CF;Gong SS
囊泡谷氨酸转运体3（VGLUT 3）在听力中起重要作用，VGLUT 3基因敲除小鼠出现耳聋。然而，VGLUT 3在耳蜗中发挥其作用的机制尚未完全确立。阐明VGLUT 3在耳蜗中的定位和表达的发育和衰老动力学将有助于对听觉神经元传递的功能理解。在这项研究中，我们的特点是表达VGLUT 3在大鼠听觉上皮和螺旋神经节细胞（SGCs）在出生后的发展和年龄，使用免疫组织化学和定量实时逆转录聚合酶链反应（RT-PCR）。VGLUT 3在耳蜗内毛细胞（IHCs）和SGCs中的表达早在出生后，随着年龄的增长，其表达逐渐增加。然而，与出生后60天（P60）相比，VGLUT 3在老化大鼠的IHC中的表达降低，并且VGLUT 3仍然在老化大鼠的剩余SGCs中表达。VGLUT 3免疫阳性点状结构仅在P7或更晚时在SGCs的细胞质中发现，而不是IHC。真实的时间定量RT-PCR检测VGLUT 3 mRNA表达的变化与免疫组化结果一致，但在整个耳蜗水平上，P60与衰老大鼠之间无统计学差异。我们的数据表明，VGLUT 3可能在大鼠耳蜗出生后的发育以及以后的生活中具有发育和生理作用。VGLUT 3除了谷氨酸转运外，可能在SGCs中具有其他未知的作用。
Vesicular glutamate transporter 3 (VGLUT3) plays an important role in hearing, andVGLUT3knockout mice are deaf. However, the mechanisms whereby VGLUT3 exerts its effects in the cochlea are not well established. Elucidating the developmental and aging dynamics of VGLUT3 localization and expression in the cochlea would aid a functional understanding of auditory glutamatergic transmission. In this study, we characterized the expression of VGLUT3 in rat auditory epithelium and spiral ganglion cells (SGCs) during postnatal development and with age using immunohistochemistry and quantitative real-time reverse transcription polymerase chain reaction (RT-PCR). We observed VGLUT3 expression in cochlea inner hair cells (IHCs) and SGCs as early as postnatally, and its expression increased gradually with maturity. However, the expression of VGLUT3 in the IHCs decreased in the aging rats compared to postnatal day 60 (P60), and VGLUT3 was still expressed in the remaining SGCs in the aging rats. VGLUT3-immunopositive punctate structures were only found in the cytoplasm of SGCs, not IHCs, at P7, or later. Variation inVGLUT3mRNA expression evaluated by quantitative real time RT-PCR was consistent with the immunohistochemical data except for no statistical difference between P60 and aging rats in the whole cochlea level. Our data suggest that VGLUT3 likely has developmental and physiological roles in the rat cochlea during postnatal development as well as later in life. VGLUT3 may have additional unknown roles in SGCs besides glutamate transport.
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