Effects of polyinosinic-polycytidylic acid and adoptive transfer of immune cells in the Lew.1AR1-iddm rat and in its coisogenic LEW.1AR1 background strain.
Effects of polyinosinic-polycytidylic acid and adoptive transfer of immune cells in the Lew.1AR1-iddm rat and in its coisogenic LEW.1AR1 background strain.
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聚肌苷-聚胞苷酸对 Lew.1AR1-iddm 大鼠及其同基因 LEW.1AR1 背景株中免疫细胞过继转移的影响。
DOI：
10.1080/08916930500114321
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发表时间：
2005
期刊：
影响因子：
3.5
通讯作者：
Hedrich,Hans-Jürgen
中科院分区：
文献类型：
--
作者：
Wedekind,Dirk;Weiss,Heike;Jörns,Anne;Lenzen,Sigurd;Tiedge,Markus;Hedrich,Hans-Jürgen
通过使用聚肌胞苷酸（Poly I：C）和过继转移来自糖尿病LEW.1AR1-iddm大鼠和共基因LEW.1AR1背景品系的伴刀豆球蛋白A（Con A）激活的淋巴细胞，研究了细胞免疫系统对LEW.1AR1-iddm大鼠发生T1 DM的重要性。Poly I：C治疗诱导糖尿病，其特征在于胰腺的弥漫性浸润，在高达20%的共基因LEW. 1AR 1背景菌株的动物中。它没有增加LEW.1AR1-iddm菌株30%的糖尿病发病率。相比之下，Poly I：C治疗在高达80%的Mhc同源LEW.1WR1菌株的动物中诱导糖尿病。在糖尿病易感的LEW.1AR1-iddm受体中，来自糖尿病供体的T细胞有丝分裂原Con A激活的淋巴细胞的连续转移使糖尿病的发病率加倍，糖尿病的形态学特征为局灶性胰岛炎。相比之下，LEW.1AR1背景品系的动物在过继转移后未发生糖尿病。此外，过继转移Con A活化的淋巴细胞从LEW.1AR 1大鼠到LEW.1AR 1-iddm大鼠（30或60%的糖尿病发病率），显著降低LEW.1AR 1-iddm大鼠（60%的糖尿病发病率）的糖尿病发病率。结果显示，自身反应性淋巴细胞诱导LEW.1AR1-iddmrat中的β细胞破坏，而LEW.1AR1背景菌株显然含有调节潜力，其能够抵消自身免疫应答。
The importance of the cellular immune system for the development of T1DM in the LEW.1AR1-iddmrat was investigated by use of polyinosinic-polycytidylic acid (Poly I:C) and by adoptive transfer of concanavalin A (Con A) activated lymphocytes from diabetic LEW.1AR1-iddmrats and the coisogenic LEW.1AR1 background strain. Poly I:C treatment induced diabetes, characterized morphologically by a diffuse infiltration of the pancreas, in up to 20% of the animals of the coisogenic LEW.1AR1 background strain. It did not increase the diabetes incidence of 30% of the LEW.1AR1-iddmstrain. In contrast Poly I:C treatment induced diabetes in up to 80% of the animals of the Mhc congenic LEW.1WR1 strain. Adoptive transfer of lymphocytes activated by the T-cell mitogen Con A from diabetic donors doubled the incidence of diabetes, characterized morphologically by a focal insulitis, in diabetes prone LEW.1AR1-iddmrecipients. In contrast, animals of the LEW.1AR1 background strain did not develop diabetes after adoptive transfer. Moreover, adoptive transfer of Con A activated lymphocytes from LEW.1AR1 rats to LEW.1AR1-iddmrats with 30 or 60% diabetes incidence, significantly decreased the incidence of diabetes in LEW.1AR1-iddmrats with 60% diabetes incidence. The results show that autoreactive lymphocytes induce beta cell destruction in the LEW.1AR1-iddmrat, while the LEW.1AR1 background strain apparently contains regulatory potential, which is able to counteract the autoimmune response.
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影响因子：
3.9
作者：
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通讯作者：
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影响因子：
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作者：
Clancy,RM;Hugli,TE
通讯作者：
Hugli,TE
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DOI：
10.1016/s0006-291x(85)80119-4
发表时间：
1985
影响因子：
3.1
作者：
M. Abe;Y. Kawazoe;H. Tsunematsu;N. Shigematsu
通讯作者：
N. Shigematsu
影响因子：
64.8
作者：
DAHLEN, SE;HEDQVIST, P;SAMUELSSON, B
通讯作者：
SAMUELSSON, B
DOI：
10.1016/s0074-7696(08)60166-7
发表时间：
1978-01-01
期刊：
International review of cytology
影响因子：
--
作者：
Kosower, N S;Kosower, E M
通讯作者：
Kosower, E M