Effects of feed contaminant deoxynivalenol on plasma cytokines and mRNA expression of immune genes in the intestine of broiler chickens.
Effects of feed contaminant deoxynivalenol on plasma cytokines and mRNA expression of immune genes in the intestine of broiler chickens.
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DOI：
10.1371/journal.pone.0071492
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Böhm J
中科院分区：
文献类型：
--
作者：
Ghareeb K;Awad WA;Soodoi C;Sasgary S;Strasser A;Böhm J
一项实验旨在研究日粮脱氧雪腐镰刀菌烯醇 (DON) 和微生物饲料添加剂对肉鸡血浆细胞因子水平和空肠组织中免疫相关基因表达的单独和联合影响。从商业孵化场获得总共 40 只肉鸡，随机分为 4 组（每组 10 只）。鸡从一天大起就在笼子里饲养，持续 5 周。饮食组是1)喂基础饮食的对照鸡； 2) DON组饲喂被10 mg DON/kg饲料污染的基础日粮； 3) DON+Mycofix组饲喂被10 mg DON/kg饲料污染的基础日粮，并补充商业饲料添加剂Mycofix® Select (MS)（2.5 kg/吨饲料）； 4)Mycofix组饲喂添加MS的基础日粮(2.5kg/吨饲料)。 35 天时，通过 ELISA 检测试剂盒对肿瘤坏死因子 α (TNF-α) 和白细胞介素 8 (IL-8) 的血浆水平进行定量。此外，通过qRT-PCR对空肠中TNF-α、IL-8、IL-1β、干扰素γ（IFNγ）、转化生长因子β受体I（TGFBR1）和活化B细胞核因子κ轻链增强子1（NF-κβ1）的mRNA表达进行定量。结果表明，DON 导致血浆 TNF-α 下降，而与 MS 联合使用时，DON 的作用减弱。 DON 下调了 IL-1β、TGFBR1 和 IFN-γ 的相对基因表达，并且在受 DON 污染的饮食中添加 MS 可补偿这些对 IL-1β、TGFBR1 的影响，但不能补偿对 IFN-γ 的影响。此外，在 DON 污染或对照饮食中补充 MS 会上调 NF-κβ1 的 mRNA 表达。总之，DON 有可能激发和调节肉鸡的免疫反应，从而增加其对疾病的易感性。这种添加剂的作用似乎与呕吐毒素几乎一样大，尽管是针对不同的基因。
An experiment was conducted to investigate the individual and combined effects of dietary deoxynivalenol (DON) and a microbial feed additive on plasma cytokine level and on the expression of immune relevant genes in jejunal tissues of broilers. A total of 40 broiler chicks were obtained from a commercial hatchery and divided randomly into four groups (10 birds per group). Birds were reared in battery cages from one day old for 5 weeks. The dietary groups were 1) control birds fed basal diet; 2) DON group fed basal diet contaminated with 10 mg DON/ kg feed; 3) DON + Mycofix group fed basal diet contaminated with 10 mg DON/ kg feed and supplemented with a commercial feed additive, Mycofix® Select (MS) (2.5 kg/ton of feed); 4) Mycofix group fed basal diet supplemented with MS (2.5 kg/ton of feed). At 35 days, the plasma levels of tumor necrosis factor alpha (TNF-α) and interleukin 8 (IL-8) were quantified by ELISA test kits. Furthermore, the mRNA expression of TNF-α, IL-8, IL-1β, interferon gamma (IFNγ), transforming growth factor beta receptor I (TGFBR1) and nuclear factor kappa-light-chain-enhancer of activated B cells 1 (NF-κβ1) in jejunum were quantified by qRT-PCR. The results showed that the plasma TNF-α decreased in response to DON, while in combination with MS, the effect of DON was reduced. DON down-regulated the relative gene expression of IL-1β, TGFBR1 and IFN-γ, and addition of MS to the DON contaminated diet compensates these effects on IL-1β, TGFBR1 but not for IFN-γ. Furthermore, supplementation of MS to either DON contaminated or control diet up-regulated the mRNA expression of NF-κβ1. In conclusion, DON has the potential to provoke and modulate immunological reactions of broilers and subsequently could increase their susceptibility to disease. The additive seemed to have almost as much of an effect as DON, albeit on different genes.
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影响因子：
3.8
作者：
Bouhet, S;Hourcade, E;Oswald, IP
通讯作者：
Oswald, IP
影响因子：
4.4
作者：
Li, Z.;Yang, Z. B.;Wu, Y. B.
通讯作者：
Wu, Y. B.
DOI：
10.1051/animres:2006032
发表时间：
2006-11-01
期刊：
ANIMAL RESEARCH
影响因子：
--
作者：
Cheng, Yeong-Hsiang;Weng, Ching-Feng;Chang, Ming-Huang
通讯作者：
Chang, Ming-Huang
影响因子：
2.8
作者：
HOLT, PS;BUCKLEY, S;DELOACH, JR
通讯作者：
DELOACH, JR
影响因子：
4.4
作者：
Awad, W. A.;Ghareeb, K.;Boehm, J.
通讯作者：
Boehm, J.