Transcriptomic events involved in melon mature-fruit abscission comprise the sequential induction of cell-wall degrading genes coupled to a stimulation of endo and exocytosis.
Transcriptomic events involved in melon mature-fruit abscission comprise the sequential induction of cell-wall degrading genes coupled to a stimulation of endo and exocytosis.
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DOI：
10.1371/journal.pone.0058363
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Gomez-Jimenez MC
中科院分区：
文献类型：
--
作者：
Corbacho J;Romojaro F;Pech JC;Latché A;Gomez-Jimenez MC
肉质果实成熟果脱落(MFA)是一个受遗传控制的过程，其机制与未成熟果脱落相反，尚未得到充分的表征。在这里，我们利用焦磷酸测序技术对甜瓜脱落区(AZ)细胞分离过程中三个阶段的转录本进行表征，以了解AZ激活早期MFA的调控。结果表明，在MFA诱导的早期，甜瓜AZ表现出主基因的诱导，而在MFA的诱导后期，甜瓜AZ表现出主基因的抑制。虽然EXT1-EXT4、EGase1、IAA2、ERF1、AP2D15、FLC、MADS2、ERAF17、SAP5和SCL13等基因在落叶诱导的早期和后期表达规律相似，但大多数基因的表达模式不同。这意味着特定时间的事件发生在MFA期间，并强调了表征多个特定时间的脱落转录本的价值。对这些AZs基因表达的分析表明，在MFA过程中，细胞壁降解基因的顺序诱导与内分泌和胞吐相关基因的上调以及植物激素代谢和信号基因的转移有关。伴随而来的是小GTP酶和合成素的转录活性，以及可能参与MFA信号转导的微管蛋白、动力蛋白、V型ATPase和激动素样蛋白。早期事件可能由MADS-box、AP2/ERF和AUX/IAA转录因子下调，而Homeobox、锌指、bZIP和WRKY转录因子上调，而晚期事件可能由MYB转录因子上调控制。总体而言，这些数据为肉质水果的MFA提供了一个全面的观点，确定了与MFA早期诱导相关的候选基因和途径。我们全面的基因表达谱对于阐明肉质水果中MFA的基因调控网络是非常有用的。
Mature-fruit abscission (MFA) in fleshy-fruit is a genetically controlled process with mechanisms that, contrary to immature-fruit abscission, has not been fully characterized. Here, we use pyrosequencing to characterize the transcriptomes of melon abscission zone (AZ) at three stages during AZ-cell separation in order to understand MFA control at an early stage of AZ-activation. The results show that by early induction of MFA, the melon AZ exhibits major gene induction, while by late induction of MFA, melon AZ shows major gene repression. Although some genes displayed similar regulation in both early and late induction of abscission, such as EXT1-EXT4, EGase1, IAA2, ERF1, AP2D15, FLC, MADS2, ERAF17, SAP5 and SCL13 genes, the majority had different expression patterns. This implies that time-specific events occur during MFA, and emphasizes the value of characterizing multiple time-specific abscission transcriptomes. Analysis of gene-expression from these AZs reveal that a sequential induction of cell-wall-degrading genes is associated with the upregulation of genes involved in endo and exocytosis, and a shift in plant-hormone metabolism and signaling genes during MFA. This is accompanied by transcriptional activity of small-GTPases and synthaxins together with tubulins, dynamins, V-type ATPases and kinesin-like proteins potentially involved in MFA signaling. Early events are potentially controlled by down-regulation of MADS-box, AP2/ERF and Aux/IAA transcription-factors, and up-regulation of homeobox, zinc finger, bZIP, and WRKY transcription-factors, while late events may be controlled by up-regulation of MYB transcription-factors. Overall, the data provide a comprehensive view on MFA in fleshy-fruit, identifying candidate genes and pathways associated with early induction of MFA. Our comprehensive gene-expression profile will be very useful for elucidating gene regulatory networks of the MFA in fleshy-fruit.
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影响因子：
5.3
作者：
Agustí J;Merelo P;Cercós M;Tadeo FR;Talón M
通讯作者：
Talón M
影响因子：
5.4
作者：
Eduardo, I;Arús, P;Monforte, AJ
通讯作者：
Monforte, AJ
影响因子：
7.4
作者：
Burr, Christian A.;Leslie, Michelle E.;Liljegren, Sarah J.
通讯作者：
Liljegren, Sarah J.
DOI：
10.21273/jashs.122.6.748
发表时间：
1997-11-01
影响因子：
1.9
作者：
Guis, M;Botondi, R;Latche, A
通讯作者：
Latche, A
影响因子：
5.3
作者：
Deleu W;Esteras C;Roig C;González-To M;Fernández-Silva I;Gonzalez-Ibeas D;Blanca J;Aranda MA;Arús P;Nuez F;Monforte AJ;Picó MB;Garcia-Mas J
通讯作者：
Garcia-Mas J