Transcriptome profiles of yellowish-white and fuchsia colored flowers in the Rheum palmatum complex reveal genes related to color polymorphism
Transcriptome profiles of yellowish-white and fuchsia colored flowers in the Rheum palmatum complex reveal genes related to color polymorphism
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大黄复合体中黄白色和紫红色花朵的转录组图谱揭示了与颜色多态性相关的基因
DOI：
10.1007/s11103-022-01299-0
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发表时间：
2022-08
影响因子：
5.1
通讯作者：
Xumei Wang
中科院分区：
文献类型：
--
作者：
Tao Zhou;Jiangyan Sun;Yunyan Zhai;Chenxi Gao;Markus Ruhsam;Xumei Wang
花色变异普遍存在于许多植物物种中，并且已经进行了一些研究来阐明潜在的分子机制。掌叶大黄复合体有黄白色和紫红色两种花色变异，但对这一现象的研究较少。在这里，我们使用两种颜色变体的转录组测序来阐明这些颜色变体的分子和生物化学基础。两个转录组的比较确定了9641差异表达的unigenes（DEG），其中包括6477上调和3163下调基因。功能分析表明，几个DEG与花色苷合成途径相关，其表达谱与qRT-PCR验证结果一致，表明结构基因的表达水平对花色变异具有重要影响。掌状复合体结果表明，转录因子MYB、bHLH和WRKY之间的相互作用也调控了红葡萄花色素苷的合成。掌状复合体利用dN/dS比值估计选择压力表明，1106对直向同源基因经历了正选择。在这些正选择的基因中，有21个基因参与花色素苷的生物合成途径，这表明它们可能编码结构改变蛋白，并影响玫瑰花的颜色。掌状复合体
Flower color variation is ubiquitous in many plant species, and several studies have been conducted to elucidate the underlying molecular mechanism. There are two flower color variants (yellowish-white and fuchsia) in the Rheum palmatum complex, however, few studies have investigated this phenomenon. Here, we used transcriptome sequencing of the two color variants to shed light on the molecular and biochemical basis for these color morphs. Comparison of the two transcriptomes identified 9641 differentially expressed unigenes (DEGs), including 6477 up-regulated and 3163 down-regulated genes. Functional analyses indicated that several DEGs were related to the anthocyanin biosynthesis pathway, and the expression profiles of these DEGs were coincident with the qRT-PCR validation results, indicating that expression levels of structural genes have a profound effect on the color variation in the R. palmatum complex. Our results suggested that the interaction of transcription factors (MYB, bHLH and WRKY) also regulated the anthocyanin biosynthesis in the R. palmatum complex. Estimation of selection pressures using the dN/dS ratio showed that 1106 pairs of orthologous genes have undergone positive selection. Of these positively selected genes, 21 were involved in the anthocyanin biosynthetic pathway, indicating that they may encode the proteins for structural alteration and affect flower color in the R. palmatum complex.
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中药大黄中顶级地药区和非顶级地药区的遗传和化学分化特征
影响因子：
4.6
作者：
Wang X;Feng L;Zhou T;Ruhsam M;Huang L;Hou X;Sun X;Fan K;Huang M;Zhou Y;Song J
通讯作者：
Song J
影响因子：
1
作者：
Yuxia Yang;Jingjing Wang;Mao Zhihu;Guosheng Sun;Changwei Zhang
通讯作者：
Yuxia Yang;Jingjing Wang;Mao Zhihu;Guosheng Sun;Changwei Zhang
DOI：
10.1093/bioinformatics/bts565
发表时间：
2012-12-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Fu L;Niu B;Zhu Z;Wu S;Li W
通讯作者：
Li W
影响因子：
7.4
作者：
Winkel-Shirley, B
通讯作者：
Winkel-Shirley, B
DOI：
10.3390/molecules23010025
发表时间：
2017-12-22
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Li Y;Luo X;Wu C;Cao S;Zhou Y;Jie B;Cao Y;Meng H;Wu G
通讯作者：
Wu G