Paenibacillus nasutitermitis sp. nov., isolated from a termite gut.

Paenibacillus nasutitermitis sp. nov., isolated from a termite gut.
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鼻白蚁类芽孢杆菌 sp.

DOI:
10.1099/ijsem.0.000807
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发表时间:
2016-02
影响因子:
2.8
通讯作者:
Yang Hong
Yang Hong
中科院分区:
生物学3区
文献类型:
--
作者:
Wang Xue Min;Ma Shichun;Yang Shu Yan;Peng Rong;Zheng Ying;Yang Hong

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从食材高等白蚁Nasutitermes sp.的后肠中分离到一株革兰氏染色阳性、不能移动、需氧和终末内孢子形成的杆状细菌P5-1T。16S rRNA基因序列的系统发育分析表明,该菌株与黑拟芽孢杆菌CCM 7311T有较近的亲缘关系(相似性为97.5 %)。在10-40 °C(最适30 °C)和pH 5.5-9.0(最适pH 7.5)条件下生长。菌株P5-1T的DNAG+C含量为48.9 摩尔%。细胞内含有作为唯一呼吸对苯二酚的MK-7,主要脂肪酸为Anteiso-C15 : 0和iso-C15 : 0。细胞内的极性脂包括磷脂酰甘油、二磷脂酰甘油、磷脂酰乙醇胺、一种未知的磷脂、一种未知的糖脂和一种未知的氨基磷脂。细胞壁肽聚糖中的二氨基酸为中位二氨基二茂酸。根据本研究获得的系统发育、化学分类和表型数据,菌株P5-1T代表了拟青霉属的一个新种,命名为鼻翼拟青霉。11月是提议的。模式菌株为P5-1T( = CGMCC1.15178T, = NBRC111536T)。
A Gram-stain-positive, non-motile, aerobic and terminal-endospore-forming rod-shaped bacterium, strain P5-1T, was isolated from the hindgut of a wood-feeding higher termite, Nasutitermes sp. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain was closely related to Paenibacillus sepulcri CCM 7311T (97.5 % similarity). Growth was observed at 10-40 °C (optimum, 30 °C) and at pH 5.5-9.0 (optimum, pH 7.5). The DNA G+C content of strain P5-1T was 48.9 mol%. Cells contained menaquinone 7 (MK-7) as the sole respiratory quinone and the major fatty acids were anteiso-C15 : 0 and iso-C15 : 0. The cellular polar lipids comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified glycolipid and one unidentified aminophospholipid. The diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid. Based on the phylogenetic, chemotaxonomic and phenotypic data obtained within this study, strain P5-1T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus nasutitermitis sp. nov. is proposed. The type strain is P5-1T ( = CGMCC 1.15178T = NBRC 111536T).
DOI: 10.1099/00221287-44-1-41
发表时间: 1966-07
期刊: Journal of general microbiology
影响因子: --
作者:
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