Development of a magnetically aligned regenerative tissue-engineered electronic nerve interface for peripheral nerve applications.
Development of a magnetically aligned regenerative tissue-engineered electronic nerve interface for peripheral nerve applications.
复制标题
用于周围神经应用的磁对齐再生组织工程电子神经接口的研制。
DOI：
10.1016/j.biomaterials.2021.121212
复制
发表时间：
2021-12
期刊：
影响因子：
14
通讯作者：
中科院分区：
文献类型：
--
作者：
摘要周围神经损伤可使运动和感觉功能衰弱，严重的病例常常导致完全截肢。在过去的二十年里，假肢技术迅速发展，为用户提供高达20°的自由度的原始运动控制;然而，提供高运动选择性所需的神经接口技术却没有以同样的速度发展。本文介绍的工作重点是开发一种磁性对齐的再生组织工程电子神经接口（MARTEENI），该接口将聚酰亚胺“线”封装在磁性对齐的水凝胶支架内。该技术利用组织工程策略来解决传统外周神经接口的问题，包括通过神经和刚性基底的轴突采样不良。磁性模板化水凝胶用于物理支撑聚酰亚胺线，同时还促进聚酰亚胺上电极部位附近的再生。这项工作证明了磁性模板的效用，用于调整水凝胶支架的机械性能，以匹配天然神经组织的刚度，同时为体外雪旺细胞迁移提供对齐的基底。制造MARTEENI器械并植入5 mm长的大鼠坐骨神经横断模型中，以评估6周和12周时的再生情况。MARTEENI器械不会破坏组织重塑，并且显示出与聚酰亚胺基底周围的新鲜组织对照品相当的轴突密度。观察到器械在聚酰亚胺线周围的异物反应减弱。预计未来使用功能性MARTEENI设备的研究将能够以高选择性和低刺激方案记录和刺激单轴突。
ABSTRACT Peripheral nerve injuries can be debilitating to motor and sensory function, with severe cases often resulting in complete limb amputation. Over the past two decades, prosthetic limb technology has rapidly advanced to provide users with crude motor control of up to 20° of freedom; however, the nerve-interfacing technology required to provide high movement selectivity has not progressed at the same rate. The work presented here focuses on the development of a magnetically aligned regenerative tissue-engineered electronic nerve interface (MARTEENI) that combines polyimide “threads” encapsulated within a magnetically aligned hydrogel scaffold. The technology exploits tissue-engineered strategies to address concerns over traditional peripheral nerve interfaces including poor axonal sampling through the nerve and rigid substrates. A magnetically templated hydrogel is used to physically support the polyimide threads while also promoting regeneration in close proximity to the electrode sites on the polyimide. This work demonstrates the utility of magnetic templating for use in tuning the mechanical properties of hydrogel scaffolds to match the stiffness of native nerve tissue while providing an aligned substrate for Schwann cell migration in vitro. MARTEENI devices were fabricated and implanted within a 5-mm-long rat sciatic-nerve transection model to assess regeneration at 6 and 12 weeks. MARTEENI devices do not disrupt tissue remodeling and show axon densities equivalent to fresh tissue controls around the polyimide substrates. Devices are observed to have attenuated foreign-body responses around the polyimide threads. It is expected that future studies with functional MARTEENI devices will be able to record and stimulate single axons with high selectivity and low stimulation regimes.
登录
查看更多内容
影响因子：
4
作者：
Barrese JC;Aceros J;Donoghue JP
通讯作者：
Donoghue JP
影响因子：
3.4
作者：
Eto, M;Yoshikawa, H;Sakoda, S
通讯作者：
Sakoda, S
影响因子：
4.1
作者：
Dewitt, Daniel D.;Kaszuba, Stephanie N.;Stegemann, Jan P.
通讯作者：
Stegemann, Jan P.
影响因子：
--
作者：
Grinsell D;Keating CP
通讯作者：
Keating CP
周围神经ECM成分在组织工程神经构建中的作用
影响因子：
4.1
作者：
Gao, Xupeng;Wang, Yu;Peng, Jiang
通讯作者：
Peng, Jiang