Genome-wide analysis and expression of the calcium-dependent protein kinase gene family in cucumber
Genome-wide analysis and expression of the calcium-dependent protein kinase gene family in cucumber
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黄瓜钙依赖性蛋白激酶基因家族的全基因组分析和表达
DOI：
10.1007/s00438-015-1002-1
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发表时间：
2015-02
影响因子：
3.1
通讯作者：
Chen Xuehao
中科院分区：
文献类型：
--
作者：
Xu Xuewen;Liu Min;Lu Lu;He Min;Qu Wenqin;Xu Qiang;Qi Xiaohua;Chen Xuehao
钙依赖性蛋白激酶（CDPKs）是一种多功能蛋白质，其在单个基因产物内结合联合收割机钙结合和信号传导能力。目前的研究表明，CDPKs调节许多生长和发育过程以及生物和非生物胁迫反应。尽管如此，有关黄瓜（Cucumis sativusL.）中CDPK家族的特异表达模式和进化历史的知识仍有待进一步研究。仍然非常有限。因此，我们研究了黄瓜基因组序列中鉴定的19个CDPK基因的系统发育关系和表达谱，根据系统发育树和基因结构将它们分解为4个亚科。组织特异性表达谱表明，黄瓜CDPK基因参与黄瓜组织发育。基于qRT-PCR的表达分析表明，黄瓜CDPK基因广泛参与脱落酸，盐，冷，干旱，热，和洪涝响应，可能通过不同的机制。两个旁系同源物分歧后的命运也进行了研究，表明在进化过程中的亚功能化和新功能化。这些结果为葫芦科植物CDPK基因家族的功能和进化分析奠定了重要基础。
Calcium-dependent protein kinases (CDPKs) are multi-functional proteins that combine calcium-binding and signaling capabilities within a single gene product. Current studies have shown that the CDPKs regulate numerous growth and developmental processes and biotic and abiotic stress responses. Nonetheless, knowledge concerning the specific expression patterns and evolutionary history of the CDPK family in cucumber (Cucumis sativusL.) remains very limited. We, therefore, investigated the phylogenetic relationships and expression profiles of the 19CDPKgenes identified in the cucumber genome sequence, resolving them into four subfamilies based on a phylogenetic tree and gene structures. Tissue-specific expression profiles suggest that cucumberCDPKgenes are involved in cucumber tissue development. An expression analysis based on qRT-PCR indicated that cucumberCDPKgenes are extensively involved in abscisic acid, salt, cold, drought, heat, and waterlogging responses, possibly by different mechanisms. The fates of two paralogs after divergence were also investigated, suggesting subfunctionalization and neofunctionalization during evolution. These observations lay an important foundation for functional and evolutionary analyses of theCDPKgene family in cucurbitaceae species.
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影响因子：
3.7
作者：
Chen F;Fasoli M;Tornielli GB;Dal Santo S;Pezzotti M;Zhang L;Cai B;Cheng ZM
通讯作者：
Cheng ZM
影响因子：
2.6
作者：
Das R;Pandey GK
通讯作者：
Pandey GK
影响因子：
20.5
作者：
Boudsocq, Marie;Sheen, Jen
通讯作者：
Sheen, Jen
影响因子：
3.9
作者：
Xiaorong S. Zhang;Jung H. Choi
通讯作者：
Xiaorong S. Zhang;Jung H. Choi
影响因子：
4.3
作者：
Dubrovina, Alexandra S.;Kiselev, Konstantin V.;Khristenko, Valeriya S.
通讯作者：
Khristenko, Valeriya S.