The evolutionary history and diverse physiological roles of the grapevine calcium-dependent protein kinase gene family.
The evolutionary history and diverse physiological roles of the grapevine calcium-dependent protein kinase gene family.
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DOI：
10.1371/journal.pone.0080818
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Cheng ZM
中科院分区：
文献类型：
--
作者：
Chen F;Fasoli M;Tornielli GB;Dal Santo S;Pezzotti M;Zhang L;Cai B;Cheng ZM
钙依赖性蛋白激酶（CDPKs）是结合Ca 2+、ATP和蛋白质底物的分子开关，充当传感器中继器和响应器，将发育过程和环境应激产生的Ca 2+信号转化为磷酸化事件。葡萄藤（葡萄属酿酒葡萄）中CDPKs的确切功能在很大程度上是未知的。因此，我们研究了在12 x葡萄藤基因组序列中鉴定的17个CDPK基因的系统发育关系和表达谱，基于系统发育树拓扑结构和基因结构将它们分解为四个亚科。葡萄藤进化过程中的CDPKs的起源进行了表征，涉及13个扩展事件。使用54个组织和发育阶段的转录组学分析揭示了三种类型的CDPK基因表达谱：组成型（管家CDPKs），分区的功能，并普遍存在于花粉/雄蕊。我们确定了两个重复的CDPK基因，已经从管家花粉流行的功能，其起源与种子植物，表明neofunctionalization在花粉发育中的重要作用，也在无核品种的育种中的潜在价值。我们还发现，CDPKs参与了三个非生物胁迫信号通路，因此可以用来研究胁迫反应之间的串扰。
Calcium-dependent protein kinases (CDPKs) are molecular switches that bind Ca2+, ATP, and protein substrates, acting as sensor relays and responders that convert Ca2+ signals, created by developmental processes and environmental stresses, into phosphorylation events. The precise functions of the CDPKs in grapevine (Vitis vinifera) are largely unknown. We therefore investigated the phylogenetic relationships and expression profiles of the 17 CDPK genes identified in the 12x grapevine genome sequence, resolving them into four subfamilies based on phylogenetic tree topology and gene structures. The origins of the CDPKs during grapevine evolution were characterized, involving 13 expansion events. Transcriptomic analysis using 54 tissues and developmental stages revealed three types of CDPK gene expression profiles: constitutive (housekeeping CDPKs), partitioned functions, and prevalent in pollen/stamen. We identified two duplicated CDPK genes that had evolved from housekeeping to pollen-prevalent functions and whose origin correlated with that of seed plants, suggesting neofunctionalization with an important role in pollen development and also potential value in the breeding of seedless varieties. We also found that CDPKs were involved in three abiotic stress signaling pathways and could therefore be used to investigate the crosstalk between stress responses.
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DOI：
10.1098/rstb.2012.0003
发表时间：
2012-09-19
影响因子：
6.3
作者：
Lehti-Shiu, Melissa D.;Shiu, Shin-Han
通讯作者：
Shiu, Shin-Han
影响因子：
64.8
作者：
Jaillon, Olivier;Aury, Jean-Marc;Wincker, Patrick
通讯作者：
Wincker, Patrick
影响因子：
12.3
作者：
Jiao Y;Leebens-Mack J;Ayyampalayam S;Bowers JE;McKain MR;McNeal J;Rolf M;Ruzicka DR;Wafula E;Wickett NJ;Wu X;Zhang Y;Wang J;Zhang Y;Carpenter EJ;Deyholos MK;Kutchan TM;Chanderbali AS;Soltis PS;Stevenson DW;McCombie R;Pires JC;Wong GK;Soltis DE;Depamphilis CW
通讯作者：
Depamphilis CW
影响因子：
5.1
作者：
Ye, Shuifeng;Wang, Lei;Lin, Yongjun
通讯作者：
Lin, Yongjun
重复基因作为新功能化的过渡状态的亚功能化。
影响因子：
3.4
作者：
Rastogi, S;Liberles, DA
通讯作者：
Liberles, DA