MiR-205-5p Functions as a Tumor Suppressor in Gastric Cancer Cells through Downregulating FAM84B.
MiR-205-5p Functions as a Tumor Suppressor in Gastric Cancer Cells through Downregulating FAM84B.
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MiR-205-5p 通过下调 FAM84B 在胃癌细胞中发挥肿瘤抑制作用
DOI：
10.1155/2022/8267891
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发表时间：
2022
影响因子：
--
通讯作者：
Cui, XiaoPeng
中科院分区：
文献类型：
--
作者：
Chen, Xi;Zhang, Lei;Geng, JingBo;Chen, Zhong;Cui, XiaoPeng
MicroRNA (miRNA) 通过调节特定靶点参与包括胃癌 (GC) 在内的多种疾病的形成。在这里，我们探讨了 miR-205-5p 在 GC 中的功能和调控机制。通过 qRT-PCR 检测 GC 细胞中的 MiR-205-5p 水平。此外，通过CCK-8实验、集落形成、流式细胞术、划痕实验、Transwell和Western印迹评估miR-205-5p在细胞增殖、细胞凋亡、细胞周期、细胞侵袭和转移中的作用。此外，利用Starbase网站预测miR-205-5p的靶基因，并通过双荧光素酶报告基因检测进一步验证。此外，进一步研究了序列相似性家族84成员B（FAM84B）对miR-205-5p上调介导的GC的功能影响。 GC 细胞中 miR-205-5p 表达降低。 miR-205-5p 的上调抑制细胞增殖和转移，并诱导 GC 细胞凋亡和周期停滞。此外，FAM84B 被预测并证实为 miR-205-5p 的靶标，并与 miR-205-5p 负相关。从机制上讲，FAM84B 过表达部分挽救了 miR-205-5p 上调对 GC 细胞进展的功能影响。这项研究表明 miR-205-5p/FAM84B 作为治疗 GC 的新靶点的潜力。
MicroRNAs (miRNAs) participate in the formation of multiple diseases, including gastric cancer (GC), through modulating specific targets. Here, we explored the functions and regulatory mechanisms of miR-205-5p in GC. MiR-205-5p levels were detected in GC cells through qRT-PCR. Besides, the role of miR-205-5p in cell proliferation, cell apoptosis, cell cycle, cell invasion, and metastasis was assessed through CCK-8 assay, colony formation, flow cytometry, scratch assay, transwell, and western blot. Moreover, the Starbase website was used to predict the target gene of miR-205-5p, further verified by a dual-luciferase reporter assay. Furthermore, the functional effects of the family with sequence similarity 84 member B (FAM84B) on GC mediated by miR-205-5p upregulation were further investigated. MiR-205-5p expression was decreased in GC cells. Upregulation of miR-205-5p inhibited cell proliferation and metastasis and induced apoptosis and cycle arrest of GC cells. Moreover, FAM84B was predicted and confirmed as a target of miR-205-5p and negatively related to miR-205-5p. Mechanically, FAM84B overexpression partially rescued the functional effects of miR-205-5p upregulation on GC cell progression. This study suggests the potential of miR-205-5p/FAM84B as novel targets for the treatment of GC.
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基因组分析表明 FAM84B 和 NOTCH 通路与食管鳞状细胞癌的进展相关。
影响因子：
9.2
作者：
Cheng C;Cui H;Zhang L;Jia Z;Song B;Wang F;Li Y;Liu J;Kong P;Shi R;Bi Y;Yang B;Wang J;Zhao Z;Zhang Y;Hu X;Yang J;He C;Zhao Z;Wang J;Xi Y;Xu E;Li G;Guo S;Chen Y;Yang X;Chen X;Liang J;Guo J;Cheng X;Wang C;Zhan Q;Cui Y
通讯作者：
Cui Y
影响因子：
3.3
作者：
Murnane, Mary Jo;Cai, Jinguo;Shuja, Sania;McAneny, David;Willett, John B.
通讯作者：
Willett, John B.
DOI：
10.1093/jnci/90.14.1072
发表时间：
1998-07-15
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
Stein, JP;Ginsberg, DA;Cote, RJ
通讯作者：
Cote, RJ
影响因子：
5.2
作者：
Jiao, Yuwen;Yang, Haojun;Tang, Liming
通讯作者：
Tang, Liming
缺氧选择性上调阳离子通道并增加肺动脉平滑肌细胞（而非冠状动脉平滑肌细胞）中的胞质 [Ca(2 )]
影响因子：
5.5
作者：
He, Xi;Song, Shanshan;Yuan, Jason X-J
通讯作者：
Yuan, Jason X-J