Active matrix metalloproteinase-2 activity discriminates colonic mucosa, adenomas with and without high-grade dysplasia, and cancers.
Active matrix metalloproteinase-2 activity discriminates colonic mucosa, adenomas with and without high-grade dysplasia, and cancers.
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DOI：
10.1016/j.humpath.2010.08.021
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发表时间：
2011-05
期刊：
影响因子：
3.3
通讯作者：
Willett, John B.
中科院分区：
文献类型：
--
作者：
Murnane, Mary Jo;Cai, Jinguo;Shuja, Sania;McAneny, David;Willett, John B.
结直肠腺瘤的病理评估是一项复杂的任务，具有显著的观察者间差异，通常定义了腺瘤切除后监测结肠镜检查的时间表。我们的特点是活性水平的前和活性基质金属蛋白酶-2和基质金属蛋白酶-9在结直肠腺瘤和癌，在结直肠肿瘤发生的病理进展的潜在标志物。特别是内源性完全激活的基质金属蛋白酶-2，由于检测困难，在腺瘤中的研究较少。在本报告中，提取了51名个体的组织（n=119），并在明胶酶谱上进行了测定，数字标准化为纳克量的纯化活性对照。通过图形和多项式logit回归分析评估所得数据，以测试基质金属蛋白酶-2或基质金属蛋白酶-9活性是否可以区分四种不同类型的结直肠组织（正常粘膜、腺瘤伴或不伴高度异型增生和浸润性癌）。活性基质金属蛋白酶-2成功区分这些组织类别。活性基质金属蛋白酶-2的中位活性随着病理进展而逐步增加，从正常粘膜到无高度异型增生的腺瘤，再到有高度异型增生的腺瘤，最后到癌。虽然基质金属蛋白酶原-2和基质金属蛋白酶原-9的活性可以在一定程度上区分不同的组织类别，这些影响并没有贡献额外的信息。活性基质金属蛋白酶-2活性与结直肠组织病理学评估显著相关。活性基质金属蛋白酶-2的能力，区分腺瘤与高度异型增生腺瘤没有高度异型增生可能是特别有用的预测未来的结直肠癌的风险为个人，从而优化调度监测结肠镜检查。
Pathologic assessment of colorectal adenomas, a complex task with significant inter-observer variability, typically defines the scheduling of surveillance colonoscopies following removal of adenomas. We have characterized the activity levels of pro- and active matrix metalloproteinase-2 and matrix metalloproteinase-9 in colorectal adenomas and carcinomas, as potential markers of pathologic progression during colorectal tumorigenesis. Endogenous fully activated matrix metalloproteinase-2, in particular, has been studied less frequently in adenomas due to difficulties in detection. For this report, tissues (n=119) from 51 individuals were extracted and assayed on gelatin zymograms with digital standardization to nanogram quantities of purified active controls. Resulting data were assessed by graphical and multinomial logit regression analyses to test whether matrix metalloproteinase-2 or matrix metalloproteinase-9 activities could discriminate among four different types of colorectal tissue (normal mucosa, adenomas with or without high grade dysplasia and invasive carcinomas). Active matrix metalloproteinase-2 successfully discriminated among these tissue categories. Median activity for active matrix metalloproteinase-2 increased in a stepwise fashion with pathologic progression from normal mucosa to adenoma without high grade dysplasia to adenoma with high grade dysplasia to cancer. Although pro-matrix metalloproteinase-2 and pro-matrix metalloproteinase-9 activities could discriminate to some extent among tissue categories, those effects did not contribute additional information. Active matrix metalloproteinase-2 activity correlated significantly with histopathologic assessment of colorectal tissues. The ability of active matrix metalloproteinase-2 to distinguish adenomas with high grade dysplasia from adenomas without high grade dysplasia may be particularly useful in predicting future colorectal cancer risk for an individual, thus optimizing scheduling of surveillance colonoscopies.
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影响因子：
4.8
作者：
Hernandez-Barrantes, S;Toth, M;Fridman, R
通讯作者：
Fridman, R
DOI：
10.1016/s0006-291x(82)80120-4
发表时间：
1982-01-01
影响因子：
3.1
作者：
BIRKEDALHANSEN, H;TAYLOR, RE
通讯作者：
TAYLOR, RE
影响因子：
2.8
作者：
Herszenyi, Laszlo;Sipos, Ferenc;Tulassay, Zsolt
通讯作者：
Tulassay, Zsolt
影响因子：
2.8
作者：
Daniel, Piotr;Wagrowska-Danilewicz, Malgorzata;Malecka-Panas, Ewa
通讯作者：
Malecka-Panas, Ewa
影响因子：
4.6
作者：
NEWELL, KJ;WITTY, JP;MATRISIAN, LM
通讯作者：
MATRISIAN, LM