Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro
Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro
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降压药贝尼地平对小鼠MC3T3-E1细胞的体外成骨作用
DOI：
10.1631/jzus.b2000628
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发表时间：
2021-05
期刊：
影响因子：
--
通讯作者：
Wang Huiming
中科院分区：
文献类型：
--
作者：
Wang Baixiang;Yang Jiakang;Fan Lijie;Wang Yu;Zhang Chenqiu;Wang Huiming
高血压是老年人常见的全身性疾病，可同时患有多种病理性骨骼疾病，包括骨质疏松症。贝尼地平（BD）广泛用于治疗高血压，已被证明对骨代谢具有有益作用。为了证实BD的成骨作用，我们利用小鼠MC3T3-E1前成骨细胞在体外研究了其成骨功能。通过甲基噻唑基二苯基溴化四唑（MTT）测定和细胞周期测定测定MC3T3-E1细胞的增殖能力与BD浓度显着相关。通过碱性磷酸酶 (ALP) 活性测试、基质矿化结节形成、成骨基因测试和蛋白质表达分析证实，BD 治疗后，MC3T3-E1 细胞的成骨分化和成熟有所增加。此外，我们的数据表明，BMP2/Smad通路可能是BD促进成骨的部分机制，而BD可能通过OPG/RANKL/RANK（核因子κB受体激活剂（NF-κB））通路抑制破骨细胞的可能功能。 BD 在进一步研究其对骨骼状况较差的高血压患者的双重治疗作用方面具有相当大的潜力，这一假设在本研究的局限性内得到了证明。
Hypertension is a prevalent systemic disease in the elderly, who can suffer from several pathological skeletal conditions simultaneously, including osteoporosis. Benidipine (BD), which is widely used to treat hypertension, has been proved to have a beneficial effect on bone metabolism. In order to confirm the osteogenic effects of BD, we investigated its osteogenic function using mouse MC3T3-E1 preosteoblast cells in vitro. The proliferative ability of MC3T3-E1 cells was significantly associated with the concentration of BD, as measured by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and cell cycle assay. With BD treatment, the osteogenic differentiation and maturation of MC3T3-E1 cells were increased, as established by the alkaline phosphatase (ALP) activity test, matrix mineralized nodules formation, osteogenic genetic test, and protein expression analyses. Moreover, our data showed that the BMP2/Smad pathway could be the partial mechanism for the promotion of osteogenesis by BD, while BD might suppress the possible function of osteoclasts through the OPG/RANKL/RANK (receptor activator of nuclear factor- κB (NF-κB)) pathway. The hypothesis that BD bears a considerable potential in further research on its dual therapeutic effect on hypertensive patients with poor skeletal conditions was proved within the limitations of the present study.
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影响因子：
6.3
作者：
Lim LS;Fink HA;Blackwell T;Taylor BC;Ensrud KE
通讯作者：
Ensrud KE
影响因子：
2.9
作者：
B. Larijani;M. Bekheirnia;A. Soltani;Alireza Khalili-Far;H. Adibi;R. Jalili
通讯作者：
B. Larijani;M. Bekheirnia;A. Soltani;Alireza Khalili-Far;H. Adibi;R. Jalili
影响因子：
4.1
作者：
Shuman Yang;N. Nguyen;J. Eisman;Tuan V. Nguyen
通讯作者：
Shuman Yang;N. Nguyen;J. Eisman;Tuan V. Nguyen
DOI：
10.1083/jcb.96.1.191
发表时间：
1983-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Sudo H;Kodama HA;Amagai Y;Yamamoto S;Kasai S
通讯作者：
Kasai S
影响因子：
3.6
作者：
Ma, Zhong-ping;Liao, Jia-cheng;Cai, Dao-zhang
通讯作者：
Cai, Dao-zhang