The Development of Microbiota and Metabolome in Small Intestine of Sika Deer (Cervus nippon) from Birth to Weaning.
The Development of Microbiota and Metabolome in Small Intestine of Sika Deer (Cervus nippon) from Birth to Weaning.
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梅花鹿出生至断奶小肠微生物群和代谢组的发育
DOI：
10.3389/fmicb.2018.00004
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发表时间：
2018
影响因子：
5.2
通讯作者：
Li G
中科院分区：
文献类型：
--
作者：
Li Z;Wang X;Zhang T;Si H;Nan W;Xu C;Guan L;Wright AG;Li G
反刍动物胃肠道内密集多样的微生物群落在营养物质的代谢和吸收以及肠道相关免疫功能中起着关键作用。了解新生反刍动物小肠中的微生物定植是通过早期干预来操纵肠道功能的重要第一步，从而对宿主生产力和健康产生长期积极影响。然而，对生命早期小肠微生物群定植及其诱导代谢产物的认识仍然有限。在本研究中，我们研究了新生西卡鹿（Cervus nippon）从出生到断奶的第1、42和70天空肠和回肠中的微生物群和代谢组。微生物数据表明，多样性和丰富度随着年龄的增加，但在第1天观察到高度的个体差异。主坐标分析显示，在空肠和回肠的微生物群落组成在三个时间点的显着差异。盐单胞菌属的丰度，乳杆菌属，土志贺菌和拟杆菌属（Bacteroides spp.）呈下降趋势，而肠杆菌属，解纤维素菌属，尿酸杆菌属，严格意义上的梭菌1和Romboutsia spp.随着年龄的增长而显著增加。对于代谢物组，空肠和回肠中的代谢物在三个时间点彼此分离。此外，蛋氨酸，苏氨酸和腐胺的量增加，而肉豆蔻酸和十五烷酸的量随着年龄的增长，分别下降。本研究表明，随着年龄的增长，小肠中的微生物群定植和代谢组变得更加发达。这可能为发育过程中微生物-代谢组-免疫相互作用提供新的线索。
The dense and diverse community of microorganisms inhabiting the gastrointestinal tract of ruminant animals plays critical roles in the metabolism and absorption of nutrients, and gut associated immune function. Understanding microbial colonization in the small intestine of new born ruminants is a vital first step toward manipulating gut function through interventions during early life to produce long-term positive effects on host productivity and health. Yet the knowledge of microbiota colonization and its induced metabolites of small intestine during early life is still limited. In the present study, we examined the microbiota and metabolome in the jejunum and ileum of neonatal sika deer (Cervus nippon) from birth to weaning at days 1, 42, and 70. The microbial data showed that diversity and richness were increased with age, but a highly individual variation was observed at day 1. Principal coordinate analysis revealed significant differences in microbial community composition across three time points in the jejunum and ileum. The abundance of Halomonas spp., Lactobacillus spp., Escherichia–Shigella, and Bacteroides spp. tended to be decreased, while the proportion of Intestinibacter spp., Cellulosilyticum spp., Turicibacter spp., Clostridium sensu stricto 1 and Romboutsia spp. was significantly increased with age. For metabolome, metabolites separated from each other across the three time points in both jejunum and ileum. Moreover, the amounts of methionine, threonine, and putrescine were increased, while the amounts of myristic acid and pentadecanoic acid were decreased with age, respectively. The present study demonstrated that microbiota colonization and the metabolome becomes more developed in the small intestine with age. This may shed new light on the microbiota-metabolome-immune interaction during development.
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影响因子：
8
作者：
El Aidy, Sahar;van Baarlen, Peter;Kleerebezem, Michiel
通讯作者：
Kleerebezem, Michiel
影响因子：
4.4
作者：
Collado, M. Carmen;Derrien, Muriel;Isolauri, Erika
通讯作者：
Isolauri, Erika
影响因子：
3.3
作者：
Abecia, L.;Martin-Garcia, A. I.;Yanez-Ruiz, D. R.
通讯作者：
Yanez-Ruiz, D. R.
DOI：
10.1155/2014/841463
发表时间：
2014
期刊：
Archaea (Vancouver, B.C.)
影响因子：
--
作者：
Abecia L;Waddams KE;Martínez-Fernandez G;Martín-García AI;Ramos-Morales E;Newbold CJ;Yáñez-Ruiz DR
通讯作者：
Yáñez-Ruiz DR
影响因子：
7.4
作者：
Kind, Tobias;Wohlgemuth, Gert;Lee, Do Yup;Lu, Yun;Palazoglu, Mine;Shahbaz, Sevini;Fiehn, Oliver
通讯作者：
Fiehn, Oliver