An antimethanogenic nutritional intervention in early life of ruminants modifies ruminal colonization by Archaea.
An antimethanogenic nutritional intervention in early life of ruminants modifies ruminal colonization by Archaea.
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DOI：
10.1155/2014/841463
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Yáñez-Ruiz DR
中科院分区：
文献类型：
--
作者：
Abecia L;Waddams KE;Martínez-Fernandez G;Martín-García AI;Ramos-Morales E;Newbold CJ;Yáñez-Ruiz DR
这项工作的目的是研究从山羊幼崽出生时喂食产甲烷菌抑制剂是否会对瘤胃中的古菌种群产生影响，以及这种影响在以后的生活中持续存在的程度。十六只山羊生下了两个孩子。八只母兔在分娩后和超过 2 个月的时间里接受了溴氯甲烷治疗 (D+)。其他 8 只山羊未接受治疗 (D−)。两组中每只母鹿的一只幼崽都接受溴氯甲烷 (k+) 治疗 3 个月，而另一只幼崽则未经治疗 (k−)，从而形成四个实验组：D+/k+、D+/k−、D−/k+ 和 D−/k−。瘤胃样本是在断奶时、断奶后 1 个月和 4 个月（出生后 3 个月和 6 个月）以及治疗期结束时（2 个月）收集的。焦磷酸测序分析显示，儿童瘤胃中存在一种经过修改的古菌群落组成，尽管这种效应在 4 个月后并未完全持续；然而，一些不太丰富的群体在治疗组和对照组动物中仍然存在差异。在后代和成年山羊之间观察到的古菌群落组成的不同反应表明，发育中的瘤胃中发生的占据不同生态位的竞争提供了干预的潜力。
The aim of this work was to study whether feeding a methanogen inhibitor from birth of goat kids and their does has an impact on the archaeal population colonizing the rumen and to what extent the impact persists later in life. Sixteen goats giving birth to two kids were used. Eight does were treated (D+) with bromochloromethane after giving birth and over 2 months. The other 8 goats were not treated (D−). One kid per doe in both groups was treated with bromochloromethane (k+) for 3 months while the other was untreated (k−), resulting in four experimental groups: D+/k+, D+/k−, D−/k+, and D−/k−. Rumen samples were collected from kids at weaning and 1 and 4 months after (3 and 6 months after birth) and from does at the end of the treating period (2 months). Pyrosequencing analyses showed a modified archaeal community composition colonizing the rumen of kids, although such effect did not persist entirely 4 months after; however, some less abundant groups remained different in treated and control animals. The different response on the archaeal community composition observed between offspring and adult goats suggests that the competition occurring in the developing rumen to occupy different niches offer potential for intervention.
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影响因子：
11
作者：
Galand, Pierre E.;Casamayor, Emilio O.;Lovejoy, Connie
通讯作者：
Lovejoy, Connie
影响因子：
3.5
作者：
Abecia, L.;Toral, P. G.;Yanez-Ruiz, D. R.
通讯作者：
Yanez-Ruiz, D. R.
影响因子：
--
作者：
Attwood, Graeme;McSweeney, Christopher
通讯作者：
McSweeney, Christopher
影响因子：
4.4
作者：
King, Erin E.;Smith, Rachel P.;Wright, Andre-Denis G.
通讯作者：
Wright, Andre-Denis G.
影响因子：
3.3
作者：
Abecia, L.;Martin-Garcia, A. I.;Yanez-Ruiz, D. R.
通讯作者：
Yanez-Ruiz, D. R.