Whole Blood Metabolomics by (1)H NMR Spectroscopy Provides a New Opportunity To Evaluate Coenzymes and Antioxidants.
Whole Blood Metabolomics by (1)H NMR Spectroscopy Provides a New Opportunity To Evaluate Coenzymes and Antioxidants.
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DOI：
10.1021/acs.analchem.7b00171
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发表时间：
2017-04-18
影响因子：
7.4
通讯作者：
Raftery D
中科院分区：
文献类型：
--
作者：
Nagana Gowda GA;Raftery D
常规的人血液代谢组学采用血清或血浆并在其中提供丰富的代谢信息。然而，这种方法缺乏测量和评估红细胞中高浓度存在的重要代谢物如辅酶和抗氧化剂的能力。作为血清/血浆代谢组学的一个重要替代方案，我们在这里展示了一个简单的1H NMR实验可以同时测量全血提取物中的辅酶和抗氧化剂，以及最近在血清/血浆中定量的近70种代谢物[Anal. 2015，87，706 - 715]。氧化还原反应的辅酶：在一些实施方案中，基本上不需要额外的努力就可以测量氧化/还原型烟酰胺腺嘌呤二核苷酸（NAD+和NADH）和烟酰胺腺嘌呤二核苷酸磷酸（NADP+和NADPH）;能量辅酶，包括三磷酸腺苷（ATP）、二磷酸腺苷（ADP）和单磷酸腺苷（AMP）;以及抗氧化剂，氧化型和还原型谷胱甘肽（GSSG和GSH）的总和。开发了一种新方法，用于检测许多这些不稳定的物质，而不影响其他血液/血浆代谢产物。辅酶和抗氧化剂在血液NMR光谱的身份建立结合1D/2D NMR技术，化学位移数据库，pH值测量，最后，与真实的化合物加标。这是第一个研究报告的主要辅酶和抗氧化剂的鉴定和量化，同时，与其他代谢物在人体血液中使用NMR光谱的大池。考虑到辅酶和抗氧化剂的水平代表了健康和许多疾病中细胞功能的敏感指标，本文提出的NMR方法可能会开启血液代谢组学的新篇章。
Conventional human blood metabolomics employs serum or plasma and provides a wealth of metabolic information therein. However, this approach lacks the ability to measure and evaluate important metabolites such as coenzymes and antioxidants that are present at high concentrations in red blood cells. As an important alternative to serum/plasma metabolomics, we show here that a simple 1H NMR experiment can simultaneously measure coenzymes and antioxidants in extracts of whole human blood, in addition to the nearly 70 metabolites that were shown to be quantitated in serum/plasma recently [Anal. Chem. 2015, 87, 706−715]. Coenzymes of redox reactions: oxidized/reduced nicotinamide adenine dinucleotide (NAD+ and NADH) and nicotinamide adenine dinucleotide phosphate (NADP+ and NADPH); coenzymes of energy including adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP); and antioxidants, the sum of oxidized and reduced glutathione (GSSG and GSH) can be measured with essentially no additional effort. A new method was developed for detecting many of these unstable species without affecting other blood/blood plasma metabolites. The identities of coenzymes and antioxidants in blood NMR spectra were established combining 1D/2D NMR techniques, chemical shift databases, pH measurements and, finally, spiking with authentic compounds. This is the first study to report identification of major coenzymes and antioxidants and quantify them, simultaneously, with the large pool of other metabolites in human blood using NMR spectroscopy. Considering that the levels of coenzymes and antioxidants represent a sensitive measure of cellular functions in health and numerous diseases, the NMR method presented here potentially opens a new chapter in the metabolomics of blood.
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影响因子：
7.4
作者：
Gowda, G. A. Nagana;Gowda, Yashas N.;Raftery, Daniel
通讯作者：
Raftery, Daniel
影响因子：
20.1
作者：
Nakamura M;Bhatnagar A;Sadoshima J
通讯作者：
Sadoshima J
影响因子：
7.3
作者：
REGLINSKI, J;SMITH, WE;STURROCK, RD
通讯作者：
STURROCK, RD
影响因子：
1.8
作者：
Liang, Xiaorong;Yang, Lulu;Deng, Yuzhong
通讯作者：
Deng, Yuzhong
影响因子：
11.4
作者：
Bar-Or, David;Bar-Or, Raphael;Rael, Leonard T.;Brody, Edward N.
通讯作者：
Brody, Edward N.