Gold, poly(beta-amino ester) nanoparticles for small interfering RNA delivery.
Gold, poly(beta-amino ester) nanoparticles for small interfering RNA delivery.
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DOI：
10.1021/nl9009793
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发表时间：
2009-06
期刊：
影响因子：
10.8
通讯作者：
Anderson DG
中科院分区：
文献类型：
--
作者：
Lee JS;Green JJ;Love KT;Sunshine J;Langer R;Anderson DG
The safe and effective delivery of RNA therapeutics remains the major barrier to their broad clinical application. Here we develop a new nanoparticulate delivery system based on inorganic particles and biodegradable polycations. First, gold nanoparticles were modified with the hydrophilic polymer poly(ethylene glycol) (PEG), and then small interfering RNA (siRNA) was conjugated to the nanoparticles via biodegradable disulfide linkages, with ~30 strands of siRNA per nanoparticle. The particles were then coated with a library of end-modified poly(β-amino ester)s (PBAEs), previously identified as capable of facilitating intracellular DNA delivery. Nanoparticulate formulations developed here facilitate high levels of in vitro siRNA delivery, facilitating delivery as good or better than the commercially available lipid reagent, Lipofectamine 2000.
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