Longitudinal, 3D in vivo imaging of sebaceous glands by coherent anti-stokes Raman scattering microscopy: normal function and response to cryotherapy.
Longitudinal, 3D in vivo imaging of sebaceous glands by coherent anti-stokes Raman scattering microscopy: normal function and response to cryotherapy.
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DOI：
10.1038/jid.2014.293
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发表时间：
2015-01
影响因子：
6.5
通讯作者：
Evans, Conor L.
中科院分区：
文献类型：
--
作者：
Jung, Yookyung;Tam, Joshua;Jalian, H. Ray;Anderson, R. Rox;Evans, Conor L.
皮脂腺具有复杂的功能，在寻常性痤疮的发病机制中起主要作用。目前研究皮脂腺的技术在本质上大多是静态的，而皮脂腺的主要功能——通过全息分泌机制分泌皮脂——只能随着时间的推移进行评估。我们提出了一种纵向的、实时的替代方案——使用相干抗斯托克斯拉曼散射（CARS）显微镜对皮脂腺进行无标记成像，该显微镜用于选择性地可视化脂质。在小鼠耳中，CARS显微镜显示了在全息分泌过程中皮脂腺的动态变化，以及对冷却引起的腺体损伤的反应。测量了详细的腺体结构，以及单个脂肪细胞和脂肪细胞群的活跃迁移。冷却产生了皮脂细胞结构和迁移的特征性变化。本研究表明，CARS显微镜是研究皮脂腺及其相关疾病的一种很有前途的三维活体工具。
Sebaceous glands perform complex functions, and are centrally involved in the pathogenesis of acne vulgaris. Current techniques for studying sebaceous glands are mostly static in nature, whereas the gland’s main function – excretion of sebum via the holocrine mechanism – can only be evaluated over time. We present a longitudinal, real-time alternative – the in vivo, label-free imaging of sebaceous glands using Coherent Anti-Stokes Raman Scattering (CARS) microscopy, which is used to selectively visualize lipids. In mouse ears, CARS microscopy revealed dynamic changes in sebaceous glands during the holocrine secretion process, as well as in response to damage to the glands caused by cooling. Detailed gland structure, plus the active migration of individual sebocytes and cohorts of sebocytes were measured. Cooling produced characteristic changes in sebocyte structure and migration. This study demonstrates that CARS microscopy is a promising tool for studying the sebaceous gland and its associated disorders in three-dimensions in vivo.
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影响因子：
13.8
作者：
Sakamoto, Fernanda H.;Lopes, Jose Daniel;Anderson, R. Rox
通讯作者：
Anderson, R. Rox
影响因子：
2.4
作者：
Quesada-Cortes, Alicia;Campos-Munoz, Lucia;Casado-Jimenez, Mariano
通讯作者：
Casado-Jimenez, Mariano
影响因子：
3.2
作者：
Jung, Yookyung;Nichols, Alexander J.;Klein, Oliver J.;Roussakis, Emmanuel;Evans, Conor L.
通讯作者：
Evans, Conor L.
影响因子：
1.9
作者：
Rajadhyaksha, M;Anderson, RR;Webb, RH
通讯作者：
Webb, RH
影响因子：
6.5
作者：
PLEWIG, G;LUDERSCHMIDT, C
通讯作者：
LUDERSCHMIDT, C