Mouse estrous cycle identification tool and images.

Mouse estrous cycle identification tool and images.
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DOI:
10.1371/journal.pone.0035538
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发表时间:
2012
期刊:
影响因子:
3.7
通讯作者:
Taft RA
Taft RA
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Byers SL;Wiles MV;Dunn SL;Taft RA

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通过对处于发情期或发情期小鼠的鉴别,可以提高定时妊娠或假妊娠小鼠的生产效率。阴道目视观察是最快的方法,不需要特殊设备,最好只在需要识别发情前期或发情阶段时使用。应变之间的差异,特别是毛色上的差异,使得通过视觉观察准确地确定发情周期的阶段变得困难。这里展示了三种不同毛色的小鼠品系:黑色(C57BL/6J)、阿古提(CByB6F1/J)和白化(BALB/cByJ),在发情周期的每个阶段阴道口的一系列图像。当需要确定所有四个阶段(发情期、发情期、发情期和退情期)时,阴道细胞学被认为是最准确的方法。一个识别工具提出,以帮助用户在确定动情的阶段时,使用阴道细胞学。这些图像和描述是学习如何通过视觉观察或阴道细胞学来确定发情周期阶段的极好资源。
The efficiency of producing timed pregnant or pseudopregnant mice can be increased by identifying those in proestrus or estrus. Visual observation of the vagina is the quickest method, requires no special equipment, and is best used when only proestrus or estrus stages need to be identified. Strain to strain differences, especially in coat color can make it difficult to determine the stage of the estrous cycle accurately by visual observation. Presented here are a series of images of the vaginal opening at each stage of the estrous cycle for 3 mouse strains of different coat colors: black (C57BL/6J), agouti (CByB6F1/J) and albino (BALB/cByJ). When all 4 stages (proestrus, estrus, metestrus, and diestrus) need to be identified, vaginal cytology is regarded as the most accurate method. An identification tool is presented to aid the user in determining the stage of estrous when using vaginal cytology. These images and descriptions are an excellent resource for learning how to determine the stage of the estrous cycle by visual observation or vaginal cytology.
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