Characteristics of corn stover pretreated with liquid hot water and fed-batch semi-simultaneous saccharification and fermentation for bioethanol production.
Characteristics of corn stover pretreated with liquid hot water and fed-batch semi-simultaneous saccharification and fermentation for bioethanol production.
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液体热水预处理玉米秸秆补料半同步糖化发酵生产生物乙醇的特性
DOI：
10.1371/journal.pone.0095455
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Qu Y
中科院分区：
文献类型：
--
作者：
Li X;Lu J;Zhao J;Qu Y
玉米秸秆在中国是一种很有前途的生物乙醇原料。为了获得更高的乙醇浓度和更高的乙醇产量，采用液体热水预处理（LHW）和分批进料半同步糖化发酵（S-SSF）提高玉米秸秆的酶解率，促进纤维素向乙醇的生物转化。结果表明：LHW预处理玉米秸秆固体残渣在3.95 ~ 4.54的严重因子范围内可有效转化为乙醇，木聚糖的最高去除率约为89%；在初始底物浓度为10%，预水解时间为6 h，固体残渣添加量为6.1%的条件下，分批进料S-SSF的乙醇浓度分别为38.4 g/L和39.4 g/L，乙醇产率分别为78.6%和79.7%，严重因子为3.95和4.54。分析了玉米秸秆经LHW处理后的表面形态结构、比表面积、孔隙体积和直径的变化，以解释可能的改善机制。
Corn stover is a promising feedstock for bioethanol production because of its abundant availability in China. To obtain higher ethanol concentration and higher ethanol yield, liquid hot water (LHW) pretreatment and fed-batch semi-simultaneous saccharification and fermentation (S-SSF) were used to enhance the enzymatic digestibility of corn stover and improve bioconversion of cellulose to ethanol. The results show that solid residues from LHW pretreatment of corn stover can be effectively converted into ethanol at severity factors ranging from 3.95 to 4.54, and the highest amount of xylan removed was approximately 89%. The ethanol concentrations of 38.4 g/L and 39.4 g/L as well as ethanol yields of 78.6% and 79.7% at severity factors of 3.95 and 4.54, respectively, were obtained by fed-batch S-SSF in an optimum conditions (initial substrate consistency of 10%, and 6.1% solid residues added into system at the prehydrolysis time of 6 h). The changes in surface morphological structure, specific surface area, pore volume and diameter of corn stover subjected to LHW process were also analyzed for interpreting the possible improvement mechanism.
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