Age-Related Changes in the Primary Motor Cortex of Newborn to Adult Domestic Pig Sus scrofa domesticus.
Age-Related Changes in the Primary Motor Cortex of Newborn to Adult Domestic Pig Sus scrofa domesticus.
复制标题
DOI：
10.3390/ani11072019
复制
发表时间：
2021-07-06
期刊：
影响因子：
--
通讯作者：
Pirone A
中科院分区：
文献类型：
--
作者：
Desantis S;Minervini S;Zallocco L;Cozzi B;Pirone A
在过去的几十年里，使用猪脑作为神经化学研究模型的研究数量急剧增加。猪脑的关键翻译特征是它的大小和与其他哺乳动物物种的皮质和皮质下结构的相似性;从解剖学和组织学的角度来看，大脑似乎与人类相似。在这里，我们专注于在发展过程中的运动皮层，描述其细胞结构和神经细胞的分布表达两种钙结合蛋白：小清蛋白（PV）和钙视网膜蛋白（CR）。PV和CR在运动神经元的输出控制中起重要作用。本研究的形态计量学和免疫组织化学分析显示，年龄相关的变化类似于人类运动皮质中报道的变化。这些结果证实了猪脑是发育过程中有价值的转化动物模型。猪已越来越多地被用作转化神经科学的合适动物模型。然而，这个物种的快速生长，立即运动能力的大脑皮层的几个特征已经得到了充分的描述。本研究分析了新生猪、幼猪和成年猪初级运动皮层（M1）的细胞构筑。此外，我们研究了整个M1表达钙结合蛋白（CaBP）（钙视网膜蛋白，CR;小清蛋白，PV）的神经细胞的分布。新生仔猪的初级运动皮层的特点是由一个密集的神经元的安排，使歧视的细胞层困难，除了第一层。在幼猪和成年猪中观察到缺乏明显可识别的第四层，这是无颗粒皮质的典型特征。形态测定和免疫组织化学分析显示了年龄相关变化，其特征为：（1）出生后第一年内厚度增加和神经元密度（M1的细胞数/mm 2）降低;（2）出生后前几个月内CR免疫反应阳性神经元的形态学变化;（3）与年轻和成年猪相比，新生猪中CR和PV免疫反应阳性神经元的密度较高。由于目前的大多数研究结果与人类M1的结果相匹配，因此这项研究加强了越来越多的证据，即猪的大脑可以在生长和发育期间用作潜在的有价值的转化动物模型。
Over the past decades, the number of studies employing the pig brain as a model for neurochemical studies has dramatically increased. The key translational features of the pig brain are its size and similarities with the cortical and subcortical structures of other mammalian species; the brain seems to resemble a humans’ from an anatomical and histological point of view. Here we focus on the motor cortex during development, describing its cytoarchitecture and distribution of neural cells expressing two calcium-binding proteins: parvalbumin (PV) and calretinin (CR). PV and CR play an important role in the control of motor neuron output. The morphometric and immunohistochemical analysis of the present study revealed age-associated changes similar to those reported in the human motor cortex. These results confirm the pig brain is a valuable translational animal model during development. The pig has been increasingly used as a suitable animal model in translational neuroscience. However, several features of the fast-growing, immediately motor-competent cerebral cortex of this species have been adequately described. This study analyzes the cytoarchitecture of the primary motor cortex (M1) of newborn, young and adult pigs (Sus scrofa domesticus). Moreover, we investigated the distribution of the neural cells expressing the calcium-binding proteins (CaBPs) (calretinin, CR; parvalbumin, PV) throughout M1. The primary motor cortex of newborn piglets was characterized by a dense neuronal arrangement that made the discrimination of the cell layers difficult, except for layer one. The absence of a clearly recognizable layer four, typical of the agranular cortex, was noted in young and adult pigs. The morphometric and immunohistochemical analyses revealed age-associated changes characterized by (1) thickness increase and neuronal density (number of cells/mm2 of M1) reduction during the first year of life; (2) morphological changes of CR-immunoreactive neurons in the first months of life; (3) higher density of CR- and PV-immunopositive neurons in newborns when compared to young and adult pigs. Since most of the present findings match with those of the human M1, this study strengthens the growing evidence that the brain of the pig can be used as a potentially valuable translational animal model during growth and development.
登录
查看更多内容
影响因子：
2.9
作者：
Baldellon, C;Alattia, JR;Padilla, A
通讯作者：
Padilla, A
通过三重免疫染色鉴定小鼠视觉皮层中 GABA 能神经元的多种不同亚型。
影响因子：
2.9
作者：
Gonchar, Yuri;Wang, Quanxin;Burkhalter, Andreas
通讯作者：
Burkhalter, Andreas
DOI：
10.1098/rspb.1967.0002
发表时间：
1967-01-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DICKERSON, JW;DOBBING, J
通讯作者：
DOBBING, J
DOI：
10.1002/ar.a.20258
发表时间：
2005-11-01
期刊：
ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY
影响因子：
--
作者：
Hof, PR;Chanis, R;Marino, L
通讯作者：
Marino, L
影响因子：
3.3
作者：
Abrahám, H;Tóth, Z;Seress, L
通讯作者：
Seress, L