In situ enrichment of microbial communities on polarized electrodes deployed in alkaline hot springs
In situ enrichment of microbial communities on polarized electrodes deployed in alkaline hot springs
复制标题
碱性温泉中部署的极化电极上微生物群落的原位富集
DOI：
10.1016/j.jpowsour.2019.01.027
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发表时间：
2019
影响因子：
9.2
通讯作者：
Beyenal, Haluk
中科院分区：
文献类型：
--
作者：
Mohamed, Abdelrhman;Ha, Phuc T.;Peyton, Brent M.;Mueller, Rebecca;Meagher, Michelle;Beyenal, Haluk
微生物与惰性电极交换电子的能力的发现引发了基础和应用研究的新领域。然而，该领域目前仅限于在研究实验室中富集和分离的几种已知的电化学活性微生物。另一种策略是通过允许这些微生物与极化固体电极交换电子来在其原生环境中富集这些微生物。由于缺乏可用的工具，这种方法的使用目前受到限制。我们开发了一种低成本、电池供电的恒电位仪，能够控制工作电极的电位，并且可以远程部署和操作，允许在其原生环境中在电极上富集微生物。该设备在黄石国家公园心湖间歇泉盆地的四个碱性温泉中进行了测试（温度范围为45 ° C至91 ° C，pH值相对恒定为8.5-8.7）。微生物群落分析表明，在32天的极化后，微生物群落结构的变化。极化对微生物群落的影响在产生最高阴极和阳极电流的电极上最显著，表明极化对电极微生物群落有直接影响。
The discovery of the ability of microorganisms to exchange electrons with inert electrodes has triggered new areas in fundamental and applied research. However, the field is currently limited to several known electrochemically active microorganisms enriched and isolated in research laboratories. An alternative strategy is to enrich such microorganisms in their native environment by allowing them to exchange electrons with polarized solid electrodes. The use of this approach is currently limited because of a lack of available tools. We developed a low-cost, battery-operated potentiostat that is capable of controlling the potential of a working electrode and can be deployed and operated remotely, allowing the enrichment of microorganisms on electrodes in their native environment. The device was tested in four alkaline hot springs in Heart Lake Geyser Basin in Yellowstone National Park (with a temperature ranging from 45οC to 91οC and a relatively constant pH of 8.5–8.7). Microbial community analysis showed a change in microbial community structure after 32 days of polarization. The impact of polarization on microbial community was most substantial on the electrodes that generated the highest cathodic and anodic currents, suggesting a direct impact of polarization on electrode microbial community.
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影响因子：
5.7
作者：
Preiss L;Hicks DB;Suzuki S;Meier T;Krulwich TA
通讯作者：
Krulwich TA
影响因子：
14.9
作者：
Pruesse E;Quast C;Knittel K;Fuchs BM;Ludwig W;Peplies J;Glöckner FO
通讯作者：
Glöckner FO
DOI：
10.1007/978-1-4615-1831-0_1
发表时间：
1995
影响因子：
2.8
作者：
Ralph A. D. Williams;R. Sharp
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R. Sharp
影响因子：
12.6
作者：
Friedman, Elliot S.;Rosenbaum, Miriam A.;Angenent, Largus T.
通讯作者：
Angenent, Largus T.