Strain and host-cell dependent role of type-1 fimbriae in the adherence phenotype of super-shed Escherichia coli O157:H7.
Strain and host-cell dependent role of type-1 fimbriae in the adherence phenotype of super-shed Escherichia coli O157:H7.
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DOI：
10.1016/j.ijmm.2021.151511
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发表时间：
2021-05
期刊：
影响因子：
--
通讯作者：
Kapur V
中科院分区：
文献类型：
--
作者：
Katani R;Kudva IT;Srinivasan S;Stasko JB;Schilling M;Li L;Cote R;DebRoy C;Arthur TM;Sokurenko EV;Kapur V
大肠杆菌O 157（E. coliO 157）在牛直肠肛门连接部鳞状上皮（RSE）细胞上表现出强烈的聚集性肠上皮细胞消失（LEE）位点非依赖性粘附表型，并且在非LEE粘附相关位点（包括1型菌毛操纵子）中具有多态性。为了阐明1型菌毛在菌株特异性粘附和宿主特异性粘附中的作用，我们评估了4株大肠杆菌中完整的Fim操纵子（FimB-H）及其粘附（FimH）缺失突变体。coli O 157菌株、SS 17、SS 52、SS 77和EDL 933的粘附表型，并在牛RSE和人HEp-2粘附试验中评估粘附表型。与流行的教条一致，即fimH表达在E. coli O 157，Δ fimHSS 52、Δ fimB-HSS 52、Δ fimB-HSS 17和Δ fimHSS 77突变体对RSE细胞的粘附表型保持不变。相比之下，Δ fimHSS 17和Δ fimB-HSS 77突变体从野生型强和聚集性变为中等和弥漫性粘附表型，而Δ fimHEDL 933和Δ fimB-HEDL 933突变体均表现出与RSE细胞的结合增强（p < 0.05）。另外，Δ fimHSS 17和Δ fimHEDL 933均不粘附于HEp-2细胞（p < 0.05）。突变株与其各自的野生型基因互补恢复了亲本表型。显微镜检查显示，SS 17和EDL 933菌株确实携带1型菌毛样结构，短于在尿路致病性大肠杆菌中观察到的结构。杆菌总之，这些结果为fimH和fim操纵子在大肠杆菌中的菌株和宿主细胞类型依赖性作用提供了令人信服的证据。大肠杆菌O 157粘附，需要进一步评估。
Super-shed (SS) Escherichia coli O157 (E. coli O157) demonstrate a strong, aggregative, locus of enterocyte effacement (LEE)-independent adherence phenotype on bovine recto-anal junction squamous epithelial (RSE) cells, and harbor polymorphisms in non-LEE-adherence-related loci, including in the type 1 fimbriae operon. To elucidate the role of type 1 fimbriae in strain- and host-specific adherence, we evaluated the entire Fim operon (FimB-H) and its adhesion (FimH) deletion mutants in four E. coli O157 strains, SS17, SS52, SS77 and EDL933, and evaluated the adherence phenotype in bovine RSE and human HEp-2 adherence assays. Consistent with the prevailing dogma that fimH expression is genetically switched off in E. coli O157, the ΔfimHSS52, ΔfimB-HSS52, ΔfimB-HSS17, and ΔfimHSS77 mutants remained unchanged in adherence phenotype to RSE cells. In contrast, the ΔfimHSS17 and ΔfimB-HSS77 mutants changed from a wild-type strong and aggregative, to a moderate and diffuse adherence phenotype, while both ΔfimHEDL933 and ΔfimB-HEDL933 mutants demonstrated enhanced binding to RSE cells (p < 0.05). Additionally, both ΔfimHSS17 and ΔfimHEDL933 were non-adherent to HEp-2 cells (p < 0.05). Complementation of the mutant strains with their respective wild-type genes restored parental phenotypes. Microscopy revealed that the SS17 and EDL933 strains indeed carry type 1 fimbriae-like structures shorter than those seen in uropathogenic E. coli. Taken together, these results provide compelling evidence for a strain and host cell type-dependent role of fimH and the fim operon in E. coli O157 adherence that needs to be further evaluated.
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蛋白质翻译和细胞死亡：稀有TRNA在生物膜形成和激活休眠噬菌体杀手基因中的作用。
影响因子：
3.7
作者：
Garcia-Contreras, Rodolfo;Zhang, Xue-Song;Kim, Younghoon;Wood, Thomas K.
通讯作者：
Wood, Thomas K.
影响因子：
3.7
作者：
Cote, Rebecca;Katani, Robab;Kapur, Vivek
通讯作者：
Kapur, Vivek
DOI：
10.1111/j.1432-1033.1984.tb08386.x
发表时间：
1984-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
KLEMM, P
通讯作者：
KLEMM, P
影响因子：
14.9
作者：
Burland, V;Shao, Y;Blattner, FR
通讯作者：
Blattner, FR
影响因子：
3.1
作者：
Jordan, DM;Cornick, N;Moon, HW
通讯作者：
Moon, HW