Fatty acyl-CoA reductase influences wax biosynthesis in the cotton mealybug, Phenacoccus solenopsis Tinsley.

Fatty acyl-CoA reductase influences wax biosynthesis in the cotton mealybug, Phenacoccus solenopsis Tinsley.
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脂肪酰基辅酶A还原酶影响棉粉虱(Phenacoccus sonopsis Tinsley)蜡的生物合成

DOI:
10.1038/s42003-022-03956-y
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发表时间:
2022-10-19
影响因子:
5.9
通讯作者:
--
中科院分区:
生物学2区
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--
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粉蚧对多种植物都有很强的攻击性。覆盖在珠被最外层的蜡质层是这些害虫的重要保护性防御。然而,在粉蚧蜡生物合成的分子机制仍然在很大程度上未知。在这里,我们分析了棉花粉蚧,Phenacoccus solenopsis Tinsley的蜡生物合成的多组学数据,并发现,脂肪酰辅酶A还原酶(PsFAR)基因,这是高度表达的雌性粉蚧的脂肪体,有助于蜡的生物合成,通过调节生产的主要化学成分的蜡,角质烃(CHCs)。通过dsRNA显微注射对PsFAR进行RNA干扰(RNAi)并允许粉蚧以表达靶dsRNA的转基因烟草为食,导致蜡质层中CHC含量降低,并且在干燥和溴氰菊酯处理下粉蚧死亡率增加。总之,PsFAR在粉蚧的蜡生物合成中起着至关重要的作用,从而有助于它们适应水分流失和杀虫剂胁迫。
Mealybugs are highly aggressive to a diversity of plants. The waxy layer covering the outermost part of the integument is an important protective defense of these pests. However, the molecular mechanisms underlying wax biosynthesis in mealybugs remain largely unknown. Here, we analyzed multi-omics data on wax biosynthesis by the cotton mealybug, Phenacoccus solenopsis Tinsley, and found that a fatty acyl-CoA reductase (PsFAR) gene, which was highly expressed in the fat bodies of female mealybugs, contributed to wax biosynthesis by regulating the production of the dominant chemical components of wax, cuticular hydrocarbons (CHCs). RNA interference (RNAi) against PsFAR by dsRNA microinjection and allowing mealybugs to feed on transgenic tobacco expressing target dsRNA resulted in a reduction of CHC contents in the waxy layer, and an increase in mealybug mortality under desiccation and deltamethrin treatments. In conclusion, PsFAR plays crucial roles in the wax biosynthesis of mealybugs, thereby contributing to their adaptation to water loss and insecticide stress.
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