Overexpression of a Cytosolic 6-Phosphogluconate Dehydrogenase Gene Enhances the Resistance of Rice to Nilaparvata lugens.
Overexpression of a Cytosolic 6-Phosphogluconate Dehydrogenase Gene Enhances the Resistance of Rice to Nilaparvata lugens.
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DOI：
10.3390/plants9111529
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发表时间：
2020-11-10
期刊：
影响因子：
--
通讯作者：
Lou Y
中科院分区：
文献类型：
--
作者：
Chen L;Kuai P;Ye M;Zhou S;Lu J;Lou Y
磷酸戊糖途径（PPP）在植物的生长发育及植物对生物和非生物胁迫的应答中起着重要作用。然而，PPP是否调节植物对草食性昆虫的防御仍不清楚。本研究克隆了水稻胞质6-磷酸葡萄糖酸脱氢酶基因Os 6PGDH 1，该基因编码催化PPP氧化反应第三步的关键酶，并探讨了其在褐飞虱（Nilaparvata lugens）诱导的水稻防御中的作用。Os 6PGDH 1基因在5个组织中均表达，外叶鞘中表达量最高，内叶鞘中表达量最低。Os 6PGDH 1表达强烈诱导机械创伤，感染的怀孕BPH女性，茉莉酸（JA）治疗。过量表达Os 6PGDH 1（oe 6PGDH）降低了水稻株高和地上部质量，但略微增加了根长。此外，Os 6 PGDH 1的过表达增强了BPH诱导的JA、茉莉酸-异白氨酸（JA-Ile）和H2 O2的水平，但降低了BPH诱导的乙烯水平。生物测定表明，怀孕的BPH女性更喜欢饲料和产卵的野生型（WT）的植物oe 6PGDH植物，此外，孵化率的BPH卵上提出的oe 6PGDH植物和繁殖力的BPH女性喂养这些显着低于鸡蛋和女性提出和喂养野生型植物。综上所述，这些结果表明Os 6PGDH 1不仅在水稻生长中起关键作用，而且通过调节JA、乙烯和H2 O2途径在水稻对BPH的抗性中起关键作用。
The pentose phosphate pathway (PPP) plays an important role in plant growth and development, and plant responses to biotic and abiotic stresses. Yet, whether the PPP regulates plant defenses against herbivorous insects remains unclear. In this study, we cloned a rice cytosolic 6-phosphogluconate dehydrogenase gene, Os6PGDH1, which encodes the key enzyme catalyzing the third step in the reaction involving the oxidative phase of the PPP, and explored its role in rice defenses induced by brown planthopper (BPH) Nilaparvata lugens. Levels of Os6PGDH1 transcripts were detected in all five examined tissues, with the highest in outer leaf sheaths and lowest in inner leaf sheaths. Os6PGDH1 expression was strongly induced by mechanical wounding, infestation of gravid BPH females, and jasmonic acid (JA) treatment. Overexpressing Os6PGDH1 (oe6PGDH) decreased the height of rice plants and the mass of the aboveground part of plants, but slightly increased the length of plant roots. In addition, the overexpression of Os6PGDH1 enhanced levels of BPH-induced JA, jasmonoyl-isoleucine (JA-Ile), and H2O2, but decreased BPH-induced levels of ethylene. Bioassays revealed that gravid BPH females preferred to feed and lay eggs on wild-type (WT) plants over oe6PGDH plants; moreover, the hatching rate of BPH eggs raised on oe6PGDH plants and the fecundity of BPH females fed on these were significantly lower than the eggs and the females raised and fed on WT plants. Taken together, these results indicate that Os6PGDH1 plays a pivotal role not only in rice growth but also in the resistance of rice to BPH by modulating JA, ethylene, and H2O2 pathways.
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影响因子：
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作者：
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